General Stability | Organism |
---|---|
stability depends on protein concentration | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ribose 1-phosphate | competitive | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
thymidine | - |
Escherichia coli | |
0.89 | - |
phosphate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate | ordered sequential reaction mechanism, phosphate is the first substrate to bind to and deoxyribose the last product to dissociate from the enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
122 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 10 mM Tris-HCl, pH 7.3, stable for several months | Escherichia coli |
4°C, gradual loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: deoxycytidine | Escherichia coli | ? | - |
? | |
additional information | specific for deoxyribonucleosides | Escherichia coli | ? | - |
? | |
additional information | purine deoxyribonucleosides and ribonucleosides are not cleaved | Escherichia coli | ? | - |
? | |
thymidine + arsenate | - |
Escherichia coli | ? | - |
? | |
thymidine + phosphate | - |
Escherichia coli | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | two identical subunits, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Escherichia coli |
7.1 | - |
assay at | Escherichia coli |