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Literature summary for 2.4.2.39 extracted from

  • Zabotina, O.A.; van de Ven, W.T.; Freshour, G.; Drakakaki, G.; Cavalier, D.; Mouille, G.; Hahn, M.G.; Keegstra, K.; Raikhel, N.V.
    Arabidopsis XXT5 gene encodes a putative alpha-1,6-xylosyltransferase that is involved in xyloglucan biosynthesis (2008), Plant J., 56, 101-115.
    View publication on PubMed
Search for more literature for 2.4.2.39

Cloned(Commentary)

Cloned (Comment) Organism
in pENTR/D-TOPO downstream of cauliflower mosaic virus 35S promoter for recombination with pEarleyGate 201 and Agrobacterium tumefacies strain GV3101-mediated transformation (floral-flip), expression with hemagglutinin (HA) tag Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus immunolocalization of recombinant enzyme using tag-specific antibody, predicted Golgi-resident type-II membrane protein Arabidopsis thaliana 5794
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 predicted from primary structure Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-xylose + (glucosyl)xyloglucan Arabidopsis thaliana
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 UDP + (xylosyl-glucosyl)xyloglucan reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9CA75
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Source Tissue

Source Tissue Comment Organism Textmining
cauline leaf high expression level, revealed by Reverse Transcription-PCR Arabidopsis thaliana
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plant expressed in all major organs, revealed by Reverse Transcription-PCR Arabidopsis thaliana
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root high expression level, revealed by Reverse Transcription-PCR, shorter root hair and less root tissue xyloglucan detectable upon lack of XXT5 Arabidopsis thaliana
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seed vessel silique, lowest expression level, revealed by Reverse Transcription-PCR Arabidopsis thaliana
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stem high expression level, revealed by Reverse Transcription-PCR Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no xylosyltransferase activity in vitro after expression in either Pichia pastoris or Sf21 insect cells using UDP-xylose and a broad range of putative acceptors Arabidopsis thaliana ?
-
 ?
UDP-D-xylose + (glucosyl)xyloglucan
-
 Arabidopsis thaliana UDP + (xylosyl-glucosyl)xyloglucan reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5 ?

Synonyms

Synonyms Comment Organism
At1g74380
-
 Arabidopsis thaliana
XXT5 putative xyloglycan xylosylransferase Arabidopsis thaliana