Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GDP-D-glucose | activation | Glycine max | |
GDP-D-glucose | about half as effective as UDPglucose, in concentrations exceeding UDPxylose | Glycine max | |
TDPglucose | activation, as effective as UDPglucose, in concentrations exceeding UDPxylose | Glycine max | |
UDPglucose | activation, xylose is effectively incorporated in the presence of UDPglucose, the transfer must be preceded by elongation of the beta-1,4-glucan-backbone, because xylosyl residues constitute the side chains, in concentrations exceeding UDPxylose, no activation by CDPglucose or ADPglucose | Glycine max |
General Stability | Organism |
---|---|
DTT, 1 mM, EDTA, 1 mM, sucrose, 0.4 M, bovine serum albumin, 0.1%, stabilize | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Detergents | no solubilization possible due to this inhibition | Glycine max | |
EDTA | weak | Glycine max | |
additional information | no inhibition by tunicamycin, ATP or GTP | Glycine max | |
phosphate buffer | - |
Glycine max | |
Tris-HCl buffer | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Glycine max | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation, can replace Mn2+ to some extent | Glycine max | |
Mn2+ | activation | Glycine max | |
Mn2+ | 10 mM | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-xylose + xyloglucan | Glycine max | responsible for xyloglucan side-chain formation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soy bean | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-xylose + (glucosyl)xyloglucan | no acceptors are cello-oligosaccharides and fragment oligosaccharides from endoglucanase digest | Glycine max | UDP + (xylosyl-glucosyl)xyloglucan | forms an alpha-1,6-D-xylosyl-D-glucose linkage | ? | |
UDP-D-xylose + (glucosyl)xyloglucan | other xylosyl-acceptors are beta-1,3-glucan and xylan | Glycine max | UDP + (xylosyl-glucosyl)xyloglucan | forms an alpha-1,6-D-xylosyl-D-glucose linkage | ? | |
UDP-D-xylose + (glucosyl)xyloglucan | transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan | Glycine max | UDP + (xylosyl-glucosyl)xyloglucan | forms an alpha-1,6-D-xylosyl-D-glucose linkage | ? | |
UDP-D-xylose + xyloglucan | responsible for xyloglucan side-chain formation | Glycine max | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
incorporation of xylosyl residues into polymeric acceptors | Glycine max |
6.5 | 7 | UDPglucose + UDPxylose | Glycine max |