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Literature summary for 2.4.2.39 extracted from

  • Hayashi, T.; Matsuda, K.
    Biosynthesis of xyloglucan in suspension-cultured soybean cells. Occurrence and some properties of xyloglucan 4-beta-D-glucosyltransferase and 6-alpha-D-xylosyltransferase (1981), J. Biol. Chem., 256, 11117-11122.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GDP-D-glucose activation Glycine max
GDP-D-glucose about half as effective as UDPglucose, in concentrations exceeding UDPxylose Glycine max
TDPglucose activation, as effective as UDPglucose, in concentrations exceeding UDPxylose Glycine max
UDPglucose activation, xylose is effectively incorporated in the presence of UDPglucose, the transfer must be preceded by elongation of the beta-1,4-glucan-backbone, because xylosyl residues constitute the side chains, in concentrations exceeding UDPxylose, no activation by CDPglucose or ADPglucose Glycine max

General Stability

General Stability Organism
DTT, 1 mM, EDTA, 1 mM, sucrose, 0.4 M, bovine serum albumin, 0.1%, stabilize Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Detergents no solubilization possible due to this inhibition Glycine max
EDTA weak Glycine max
additional information no inhibition by tunicamycin, ATP or GTP Glycine max
phosphate buffer
-
Glycine max
Tris-HCl buffer
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Glycine max 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation, can replace Mn2+ to some extent Glycine max
Mn2+ activation Glycine max
Mn2+ 10 mM Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-xylose + xyloglucan Glycine max responsible for xyloglucan side-chain formation ?
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soy bean
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-xylose + (glucosyl)xyloglucan no acceptors are cello-oligosaccharides and fragment oligosaccharides from endoglucanase digest Glycine max UDP + (xylosyl-glucosyl)xyloglucan forms an alpha-1,6-D-xylosyl-D-glucose linkage ?
UDP-D-xylose + (glucosyl)xyloglucan other xylosyl-acceptors are beta-1,3-glucan and xylan Glycine max UDP + (xylosyl-glucosyl)xyloglucan forms an alpha-1,6-D-xylosyl-D-glucose linkage ?
UDP-D-xylose + (glucosyl)xyloglucan transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan Glycine max UDP + (xylosyl-glucosyl)xyloglucan forms an alpha-1,6-D-xylosyl-D-glucose linkage ?
UDP-D-xylose + xyloglucan responsible for xyloglucan side-chain formation Glycine max ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
incorporation of xylosyl residues into polymeric acceptors Glycine max
6.5 7 UDPglucose + UDPxylose Glycine max