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Literature summary for 2.4.2.37 extracted from
- Effects of over-expression of the regulatory enzymes DraT and DraG on the ammonium-dependent post-translational regulation of nitrogenase reductase in Azospirillum brasilense (2005), Arch. Microbiol., 183, 209-217.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional overexpression in Escherichia coli as N-terminally His6-tagged protein, leading to NifH Fe-Protein modification in absence of ammonium, i.e. the recombinant His-tagged enzyme loses its regulation | Azospirillum brasilense |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + [dinitrogen reductase] | Azospirillum brasilense | enzyme is involved in regulation of nitrogen fixation, enzyme acts together with the dinitrogenase reductase glycohydrolase DraG in posttranslational modification of the nitrogenase involving NH4Cl, overview, regulation of the enzyme, overview | nicotinamide + ADP-D-ribosyl-[dinitrogen reductase] | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azospirillum brasilense | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Azospirillum brasilense |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Azospirillum brasilense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + [dinitrogen reductase] | - |
Azospirillum brasilense | nicotinamide + ADP-D-ribosyl-[dinitrogen reductase] | - |
r | |
| NAD+ + [dinitrogen reductase] | enzyme is involved in regulation of nitrogen fixation, enzyme acts together with the dinitrogenase reductase glycohydrolase DraG in posttranslational modification of the nitrogenase involving NH4Cl, overview, regulation of the enzyme, overview | Azospirillum brasilense | nicotinamide + ADP-D-ribosyl-[dinitrogen reductase] | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dinitrogenase reductase ADP-ribosyltransferase | - |
Azospirillum brasilense |
| DRAT | - |
Azospirillum brasilense |
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Release 2023.1 (January 2023)
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