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Literature summary for 2.4.2.37 extracted from

  • Lowery, R.G.; Ludden, P.W.
    Purification and properties of dinitrogenase reductase ADP-ribosyltransferase from the photosynthetic bacterium Rhodospirillum rubrum (1988), J. Biol. Chem., 263, 16714-16719.
    View publication on PubMed

General Stability

General Stability Organism
ADP and NaCl stabilize during purification and storage Rhodospirillum rubrum
desalting procedures during purification inactivate Rhodospirillum rubrum
DTT stabilizes during purification Rhodospirillum rubrum
extremely unstable in crude extract or partially purified preparation Rhodospirillum rubrum
one freeze-thawing cycle leads to 50% loss of activity, ADP stabilizes Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
3-aminobenzamide
-
Rhodospirillum rubrum
KCl reversible Rhodospirillum rubrum
NaBr strong Rhodospirillum rubrum
NaCl reversible Rhodospirillum rubrum
NADH
-
Rhodospirillum rubrum
NADP+
-
Rhodospirillum rubrum
nicotinamide
-
Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
NAD+
-
Rhodospirillum rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Rhodospirillum rubrum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
1 * 29000, SDS-PAGE Rhodospirillum rubrum
30900
-
gel filtration Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + [dinitrogen reductase] Rhodospirillum rubrum involved in regulation of nitrogenase EC 1.18.6.1 activity ?
-
?

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.077
-
-
Rhodospirillum rubrum

Storage Stability

Storage Stability Organism
-80°C, in liquid N2 Rhodospirillum rubrum
0°C, in 0.2 M NaCl and 1 mM ADP, 18 h Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
etheno-NAD+ + [dinitrogen reductase]
-
Rhodospirillum rubrum ?
-
?
NAD+ + [dinitrogen reductase] high specificity for acceptor substrate, the only acceptor is dinitrogen reductase Rhodospirillum rubrum nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
?
NAD+ + [dinitrogen reductase] involved in regulation of nitrogenase EC 1.18.6.1 activity Rhodospirillum rubrum ?
-
?
nicotinamide guanine dinucleotide + [dinitrogen reductase]
-
Rhodospirillum rubrum ?
-
?
nicotinamide hypoxanthine dinucleotide + [dinitrogen reductase]
-
Rhodospirillum rubrum ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Rhodospirillum rubrum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodospirillum rubrum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rhodospirillum rubrum