General Stability | Organism |
---|---|
ADP and NaCl stabilize during purification and storage | Rhodospirillum rubrum |
desalting procedures during purification inactivate | Rhodospirillum rubrum |
DTT stabilizes during purification | Rhodospirillum rubrum |
extremely unstable in crude extract or partially purified preparation | Rhodospirillum rubrum |
one freeze-thawing cycle leads to 50% loss of activity, ADP stabilizes | Rhodospirillum rubrum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-aminobenzamide | - |
Rhodospirillum rubrum | |
KCl | reversible | Rhodospirillum rubrum | |
NaBr | strong | Rhodospirillum rubrum | |
NaCl | reversible | Rhodospirillum rubrum | |
NADH | - |
Rhodospirillum rubrum | |
NADP+ | - |
Rhodospirillum rubrum | |
nicotinamide | - |
Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
NAD+ | - |
Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rhodospirillum rubrum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000, SDS-PAGE | Rhodospirillum rubrum |
30900 | - |
gel filtration | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + [dinitrogen reductase] | Rhodospirillum rubrum | involved in regulation of nitrogenase EC 1.18.6.1 activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to near homogeneity | Rhodospirillum rubrum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.077 | - |
- |
Rhodospirillum rubrum |
Storage Stability | Organism |
---|---|
-80°C, in liquid N2 | Rhodospirillum rubrum |
0°C, in 0.2 M NaCl and 1 mM ADP, 18 h | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
etheno-NAD+ + [dinitrogen reductase] | - |
Rhodospirillum rubrum | ? | - |
? | |
NAD+ + [dinitrogen reductase] | high specificity for acceptor substrate, the only acceptor is dinitrogen reductase | Rhodospirillum rubrum | nicotinamide + ADP-D-ribosyl-[dinitrogen reductase] | - |
? | |
NAD+ + [dinitrogen reductase] | involved in regulation of nitrogenase EC 1.18.6.1 activity | Rhodospirillum rubrum | ? | - |
? | |
nicotinamide guanine dinucleotide + [dinitrogen reductase] | - |
Rhodospirillum rubrum | ? | - |
? | |
nicotinamide hypoxanthine dinucleotide + [dinitrogen reductase] | - |
Rhodospirillum rubrum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, SDS-PAGE | Rhodospirillum rubrum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodospirillum rubrum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodospirillum rubrum |