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Literature summary for 2.4.2.31 extracted from

  • Sung, V.; Tsai, C.
    ADP-ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates (2014), BMC Biochem., 15, 26.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli LC1062 cells Vibrio cholerae

Protein Variants

Protein Variants Comment Organism
E579Q the mutant shows reduced biotinylation intensity compared to the wild type enzyme Vibrio cholerae
E581Q the mutant exhibits strongly reduced biotinylation intensity compared to the wild type enzyme Vibrio cholerae
R426A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R426K/R451K/R473K/R479K/R506K/R519K/R522K/R525K/R535K/R540K/R543K/R566K/R629K the mutant shows no auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R451K/R473K/R479K/R506K/R519K/R522K/R535K/R540K/R543K/R566K/R629K the mutant shows no auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R451K/R473K/R506K/R519K/R522K/R525K/R535K/R540K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and severely reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R451K/R473K/R506K/R519K/R522K/R535K/R540K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R451K/R473K/R506K/R519K/R522K/R540K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R473K/R479K/R506K/R519K/R522K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R473K/R506K/R519K/R522K/R540K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R473K/R506K/R519K/R522K/R540K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R473K/R506K/R519K/R522K/R543K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R473K/R506K/R519K/R522K/R566K/R629K the mutant shows increased auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R426K/R473K/R506K/R566K/R629K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R451A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R473A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R479A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R506A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R506K the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R506K/R566K the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme Vibrio cholerae
R506Q the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R519A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R522A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R525A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R525K the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R525Q the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R530A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R530K inactive Vibrio cholerae
R530Q inactive Vibrio cholerae
R535A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R540A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R543A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R566A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
R629A the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme Vibrio cholerae
Y493A inactive Vibrio cholerae
Y493A/E579Q/E581Q inactive Vibrio cholerae
Y493A/E581Q inactive Vibrio cholerae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Vibrio cholerae the enzyme also shows auto-ADP-ribosylation activity ?
-
 ?
NAD+ + protein L-arginine Vibrio cholerae
-
 nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae Q5EK40
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Vibrio cholerae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also shows auto-ADP-ribosylation activity Vibrio cholerae ?
-
 ?
NAD+ + eEF2 L-arginine
-
 Vibrio cholerae nicotinamide + Nomega-(ADP-D-ribosyl)-eEF2-L-arginine
-
 ?
NAD+ + protein L-arginine
-
 Vibrio cholerae nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
 ?
NAD+ + protein poly-L-arginine
-
 Vibrio cholerae nicotinamide + Nomega-(ADP-D-ribosyl)-protein-poly-L-arginine
-
 ?
NAD+ + protein R10H6 L-arginine
-
 Vibrio cholerae nicotinamide + Nomega-(ADP-D-ribosyl)-protein N10H6-L-arginine
-
 ?

Synonyms

Synonyms Comment Organism
ADPRT
-
 Vibrio cholerae
cholix toxin
-
 Vibrio cholerae