Literature summary for 2.4.2.30 extracted from

Yasmin, L.; Jansson, A.L.; Panahandeh, T.; Palmer, R.H.; Francis, M.S.; Hallberg, B.
Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity (2006), FEBS J., 273, 638-646.

Search for more literature for 2.4.2.30

Activating Compound

Activating Compound	Comment	Organism	Structure
protein 14-3-3	required, interaction analysis, interaction equires residues L426, D427, and L428, overview, binding study of mutant enzymes, overview	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
expression of various GST-fusion protein constructs of ExoS mutants in HeLa cells	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
A425K	site-directed mutagenesis, phenotypic analysis	Pseudomonas aeruginosa
D424A	site-directed mutagenesis, phenotypic analysis	Pseudomonas aeruginosa
D424A/D427A	site-directed mutagenesis, reduced interaction with 14-3-3, phenotypic analysis	Pseudomonas aeruginosa
D424A/L426A/D427A/L428A	site-directed mutagenesis, the mutant shows a highly reduced expression level, phenotypic analysis	Pseudomonas aeruginosa
D427A	site-directed mutagenesis, the mutant shows a reduced expression level, phenotypic analysis	Pseudomonas aeruginosa
G421A	site-directed mutagenesis, phenotypic analysis	Pseudomonas aeruginosa
L422A	site-directed mutagenesis, phenotypic analysis	Pseudomonas aeruginosa
L423A	site-directed mutagenesis, phenotypic analysis	Pseudomonas aeruginosa
L426A	site-directed mutagenesis, phenotypic analysis	Pseudomonas aeruginosa
L426A/D427A	site-directed mutagenesis, the mutant shows a highly reduced expression level and slightly reduced cytotoxicity, no interaction with 14-3-3, phenotypic analysis	Pseudomonas aeruginosa
L426A/D427A/L428A	site-directed mutagenesis, the mutant shows a highly reduced expression level and cytotoxicity, no interaction with 14-3-3, phenotypic analysis	Pseudomonas aeruginosa
L428A	site-directed mutagenesis, reduced interaction with 14-3-3, the mutant shows highly reduced cytotoxicity, phenotypic analysis	Pseudomonas aeruginosa
Q420A	site-directed mutagenesis, phenotypic analysis	Pseudomonas aeruginosa
S419I	site-directed mutagenesis, phenotypic analysis	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Pseudomonas aeruginosa	the bacterial enzyme shows the ability to mediate cell death in the host and is a toxin, residues 426-428 are required for this activity	?	-	?
NAD+ + (ADP-D-ribosyl)n-acceptor	Pseudomonas aeruginosa	-	nicotinamide + (ADP-D-ribosyl)n+1-acceptor	-	?
NAD+ + Ras	Pseudomonas aeruginosa	interaction requires residue Leu428	nicotinamide + (ADP-D-ribosyl)-Ras	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	gene exoS	-

Reaction

Reaction	Comment	Organism	Reaction ID
NAD+ + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+	L428 is essential for activity	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the bacterial enzyme shows the ability to mediate cell death in the host and is a toxin, residues 426-428 are required for this activity	Pseudomonas aeruginosa	?	-	?
NAD+ + (ADP-D-ribosyl)n-acceptor	-	Pseudomonas aeruginosa	nicotinamide + (ADP-D-ribosyl)n+1-acceptor	-	?
NAD+ + Ras	interaction requires residue Leu428	Pseudomonas aeruginosa	nicotinamide + (ADP-D-ribosyl)-Ras	-	?

Synonyms

Synonyms	Comment	Organism
ADP-ribosyltransferase	-	Pseudomonas aeruginosa
exoenzyme S	-	Pseudomonas aeruginosa
ExoS	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)