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Literature summary for 2.4.2.30 extracted from

  • Wilde, C.; Vogelsgesang, M.; Aktories, K.
    Rho-specific Bacillus cereus ADP-ribosyltransferase C3cer cloning and characterization (2003), Biochemistry, 42, 9694-9702.
    View publication on PubMed
Search for more literature for 2.4.2.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus cereus

Protein Variants

Protein Variants Comment Organism
C19A ADP-ribosyltransferase activity is 118% of the wild-type activity Bacillus cereus
E185Q no ADP-ribosyltransferase activity Bacillus cereus
Q183E no ADP-ribosyltransferase activity Bacillus cereus
R155E ADP-ribosyltransferase activity is 0.2% of the wild-type activity Bacillus cereus
R59K ADP-ribosyltransferase activity is 1.1% of the wild-type activity Bacillus cereus
R59K/R97K no ADP-ribosyltransferase activity Bacillus cereus
R97K ADP-ribosyltransferase activity is 1.9% of the wild-type activity Bacillus cereus
T178A ADP-ribosyltransferase activity is 1.3% of the wild-type activity Bacillus cereus
T178A/Q183E no ADP-ribosyltransferase activity Bacillus cereus
Y151V ADP-ribosyltransferase activity is 1.6% of the wild-type activity Bacillus cereus
Y180T no ADP-ribosyltransferase activity Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0171
-
 NAD+ pH 7.3, 37°C, cosubstrate RhoC Bacillus cereus
0.0208
-
 NAD+ pH 7.3, 37°C, cosubstrate RhoA Bacillus cereus
0.0564
-
 NAD+ pH 7.3, 37°C, cosubstrate RhoB Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus 2339
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.25
-
-
 Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + RhoA
-
 Bacillus cereus nicotinamide (ADP-D-ribosyl)-RhoA
-
 ?
NAD+ + RhoA
-
 Bacillus cereus 2339 nicotinamide (ADP-D-ribosyl)-RhoA
-
 ?
NAD+ + RhoB
-
 Bacillus cereus nicotinamide (ADP-D-ribosyl)-RhoB
-
 ?
NAD+ + RhoB
-
 Bacillus cereus 2339 nicotinamide (ADP-D-ribosyl)-RhoB
-
 ?
NAD+ + RhoC weak activity Bacillus cereus nicotinamide (ADP-D-ribosyl)-RhoC
-
 ?
NAD+ + RhoC weak activity Bacillus cereus 2339 nicotinamide (ADP-D-ribosyl)-RhoC
-
 ?

Synonyms

Synonyms Comment Organism
ADP-ribosyltransferase C3cer
-
 Bacillus cereus

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus cereus theoretical value
-
 9.3