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Literature summary for 2.4.2.30 extracted from
- Purification and characterization of poly (ADP-ribose) synthetase from human placenta (1987), J. Biol. Chem., 262, 2352-2357.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DNA | absolute requirement | Homo sapiens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-aminobenzamide | 1 mM, 99% inhibition | Homo sapiens | |
| 3-aminobenzoic acid | 1 mM, 12% inhibition | Homo sapiens | |
| Cu2+ | - |
Homo sapiens | |
| Hg2+ | - |
Homo sapiens | |
| nicotinamide | 1 mM, 93% inhibition | Homo sapiens | |
| PCMB | complete | Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | enhances activity | Homo sapiens | |
| Mg2+ | enhances both the automodification and poly(ADP-ribosyl)ation of histone H1 | Homo sapiens | |
| Sr2+ | enhances activity | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 116000 | - |
1 * 116000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.02 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + (ADP-D-ribosyl)n-acceptor | poly(ADP-ribosyl)ation of histone H1 | Homo sapiens | nicotinamide + (ADP-D-ribosyl)n+1-acceptor | - |
? | |
| NAD+ + (ADP-D-ribosyl)n-acceptor | catalyzes poly(ADP-ribosyl)ation of the synthetase itself, automodification | Homo sapiens | nicotinamide + (ADP-D-ribosyl)n+1-acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 116000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
reaction with histone in presence of DNA, 0.1 M glycine-NaOH buffer | Homo sapiens |
| 8.7 | - |
reaction with histone in presence of DNA, 0.1 M Tris-HCl buffer | Homo sapiens |
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