Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.29 extracted from

  • Garcia, G.A.; Chervin, S.M.; Kittendorf, J.D.
    Identification of the rate-determining step of tRNA-guanine transglycosylase from Escherichia coli (2009), Biochemistry, 48, 11243-11251.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.29

Protein Variants

Protein Variants Comment Organism
D264N is inactive and incapable of forming the covalent intermediate, while maintaining the ability to bind noncovalently to RNA Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00262
-
 [RNAECYMA]-2'-deoxyguanine 34
-
 Escherichia coli
0.00287
-
 [RNAECYMA]-guanine
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged TGT purified by Ni2+ affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-aminomethyl-7-carbaguanine + [RNAECYMA]-2'-deoxyguanine 34 TGT-RNA covalent intermediate Escherichia coli guanine + [RNAECYMA]-2'-deoxy-pre Q1
-
 ?
7-aminomethyl-7-carbaguanine + [RNAECYMA]-guanine hairpin RNA corresponding to the tRNAtyr anticodon stem-loop motif, TGT-RNA covalent intermediate Escherichia coli guanine + [RNAECYMA]-pre Q1
-
 ?
7-aminomethyl-7-carbaguanine + [tRNA]-guanine the TGT-RNA covalent complex is kinetically capable of occurring on the TGT reaction pathway. Dissociation of product RNA from the enzyme is overall rate-limiting in the steady state. Role for the 2'-hydroxyl group of the ribose in the TGT reaction Escherichia coli guanine + [tRNA]-pre Q1
-
 ?

Synonyms

Synonyms Comment Organism
TGT
-
 Escherichia coli
tRNA-guanine transglycosylase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0094
-
 [RNAECYMA]-guanine
-
 Escherichia coli
0.011
-
 [RNAECYMA]-2'-deoxyguanine 34
-
 Escherichia coli