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Literature summary for 2.4.2.26 extracted from

  • Casanova, J.C.; Roch, C.; Kuhn, J.; Kleesiek, K.; Goetting, C.
    First in-gel detection and purification of human xylosyltransferase II (2009), Biochem. Biophys. Res. Commun., 379, 243-248.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
XylT-II cloned into the multiple cloning site of the pPICZ alphaA expression vector, in frame with the alpha-factor signal sequence and with a C-terminal located myc-epitope and hexa-histidine tag, expressed in Pichia pastoris strain X-33 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 XylT-I Homo sapiens
0.0061
-
biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 XylT-II Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95800
-
XylT-II, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
XylT-II purified by fractionated ammonium sulfate precipitation, heparin affinity and ion exchange chromatography, 7087fold with a final yield of 2.6% Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000003
-
XylT-II, concentrated culture supernatant Homo sapiens
0.002333
-
7087fold purified XylT-II Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-xylose + biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 catalytic efficiency of XylT-I is more than 2.4fold higher than for XylT-II Homo sapiens UDP + biotin-NH-QEEEG-(D-xylosyl)SGGGQKK(5-fluorescein)-CONH2
-
?

Synonyms

Synonyms Comment Organism
xylosyltransferase I
-
Homo sapiens
xylosyltransferase II
-
Homo sapiens
XylT-I
-
Homo sapiens
XylT-II
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0363
-
biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 XylT-I or XylT-II Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.95
-
biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 XylT-II Homo sapiens
14.52
-
biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 XylT-II Homo sapiens