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Literature summary for 2.4.2.22 extracted from

  • Arent, S.; Kadziola, A.; Larsen, S.; Neuhard, J.; Jensen, K.F.
    The extraordinary specificity of xanthine phosphoribosyltransferase from Bacillus subtilis elucidated by reaction kinetics, ligand binding, and crystallography (2006), Biochemistry, 45, 6615-6627.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the dimeric XPRTase-GMP complex was determined to 2.05 A resolution Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
GMP competitive against 5-phospho-alpha-D-ribose 1-diphosphate Bacillus subtilis
Pyrophosphate noncompetitive Bacillus subtilis
XMP competitive against 5-phospho-alpha-D-ribose 1-diphosphate Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
xanthine
-
Bacillus subtilis
0.03
-
5-phospho-alpha-D-ribose 1-diphosphate cosubstrate: xanthine Bacillus subtilis
0.281
-
guanine
-
Bacillus subtilis
1.25
-
hypoxanthine
-
Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P42085
-
-

Purification (Commentary)

Purification (Comment) Organism
purification and formation of nucleotide free, GMP and XMP saturated complexes Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-phospho-alpha-D-ribose 1-diphosphate + guanine
-
Bacillus subtilis GMP + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine
-
Bacillus subtilis IMP + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + xanthine in a sequential reaction mechanism XPRTase binds first 5-phospho-alpha-D-ribose 1-diphosphate, stabilizing its active dimeric form, and subsequently xanthine Bacillus subtilis 9-(5-phospho-beta-D-ribosyl)xanthine + diphosphate
-
?

Synonyms

Synonyms Comment Organism
XPRTase
-
Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
guanine
-
Bacillus subtilis
0.5
-
hypoxanthine
-
Bacillus subtilis
11
-
xanthine
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 substrate: xanthine Bacillus subtilis
9.5
-
substrate: hypoxanthine Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.022
-
XMP Kis Bacillus subtilis
0.033
-
GMP Kis Bacillus subtilis