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Literature summary for 2.4.2.2 extracted from

  • Panova, N.G.; Shcheveleva, E.V.; Alexeev, C.S.; Mukhortov, V.G.; Zuev, A.N.; Mikhailov, S.N.; Esipov, R.S.; Chuvikovsky, D.V.; Miroshnikov, A.I.
    Use of 4-thiouridine and 4-thiothymidine in studies on pyrimidine nucleoside phosphorylases (2004), Mol. Biol., 38, 770-776.
No PubMed abstract available
Search for more literature for 2.4.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of enzymes with uridine and thymidine phosphorylase activities in strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibitory effects between pyrimidines and analogues Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Escherichia coli
0.13
-
 4-thiouridine pH 7.5, 25°C, uridine phosphorylase activity Escherichia coli
0.25
-
 4-thiothymidine pH 7.5, 25°C, thymidine phosphorylase activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli predictive profiling of 5-fluorouracil resistance in human cancer cells, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45
-
 purified recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-thiothymidine + phosphate uridine phosphorylase activity, see also EC 2.4.2.3 Escherichia coli 4-thiothymine + alpha-D-ribose 1-phosphate
-
 r
4-thiouridine + phosphate thymidine phosphorylase activity, see also EC 2.4.2.4 Escherichia coli 4-thiouracil + alpha-D-ribose 1-phosphate
-
 r
additional information predictive profiling of 5-fluorouracil resistance in human cancer cells, overview Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
pyrimidine nucleoside phosphorylase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
49
-
 4-thiouridine pH 7.5, 25°C, uridine phosphorylase activity Escherichia coli
63
-
 4-thiothymidine pH 7.5, 25°C, thymidine phosphorylase activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli