KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
uridine | - |
Geobacillus stearothermophilus | |
0.32 | - |
5-methyluridine | - |
Geobacillus stearothermophilus | |
0.46 | - |
thymidine | - |
Geobacillus stearothermophilus | |
0.58 | - |
2'-deoxyuridine | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Geobacillus stearothermophilus |
85000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
JTS 859 | - |
Geobacillus stearothermophilus JTS 859 | - |
JTS 859 | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
99.6 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyluridine + phosphate | - |
Geobacillus stearothermophilus | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
5-methyluridine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
deoxyuridine + phosphate | 2'-deoxyuridine | Geobacillus stearothermophilus | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
deoxyuridine + phosphate | 2'-deoxyuridine | Geobacillus stearothermophilus JTS 859 | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | - |
Geobacillus stearothermophilus | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Geobacillus stearothermophilus | uracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | uracil + alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: 25 min in 20 mM potassium phosphate, 15.1 h in 20 mM potassium phosphate and 1 mM 5-methyluridine | Geobacillus stearothermophilus |
80 | - |
1 h, 20 mM potassium phosphate, complete inactivation | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 11.5 | - |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 12 | pH 6.0: about 75% of maximal activity, pH 12.0: about 80% of maximal activity | Geobacillus stearothermophilus |