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Literature summary for 2.4.2.2 extracted from

  • Hori, N.; Watanabe, M.; Yamazaki, Y.; Mikami, Y.
    Purification and characterization of thermostable pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus (1990), Agric. Biol. Chem., 54, 763-768.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
uridine
-
Geobacillus stearothermophilus
0.32
-
5-methyluridine
-
Geobacillus stearothermophilus
0.46
-
thymidine
-
Geobacillus stearothermophilus
0.58
-
2'-deoxyuridine
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Geobacillus stearothermophilus
85000
-
gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
JTS 859
-
Geobacillus stearothermophilus JTS 859
-
JTS 859
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
99.6
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyluridine + phosphate
-
Geobacillus stearothermophilus 5-methyluracil + alpha-D-ribose 1-phosphate
-
?
5-methyluridine + phosphate
-
Geobacillus stearothermophilus JTS 859 5-methyluracil + alpha-D-ribose 1-phosphate
-
?
deoxyuridine + phosphate 2'-deoxyuridine Geobacillus stearothermophilus uracil + 2-deoxy-D-ribose 1-phosphate
-
?
deoxyuridine + phosphate 2'-deoxyuridine Geobacillus stearothermophilus JTS 859 uracil + 2-deoxy-D-ribose 1-phosphate
-
?
thymidine + phosphate
-
Geobacillus stearothermophilus thymine + 2-deoxy-D-ribose 1-phosphate
-
?
thymidine + phosphate
-
Geobacillus stearothermophilus JTS 859 thymine + 2-deoxy-D-ribose 1-phosphate
-
?
uridine + phosphate
-
Geobacillus stearothermophilus uracil + alpha-D-ribose 1-phosphate
-
?
uridine + phosphate
-
Geobacillus stearothermophilus JTS 859 uracil + alpha-D-ribose 1-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
half-life: 25 min in 20 mM potassium phosphate, 15.1 h in 20 mM potassium phosphate and 1 mM 5-methyluridine Geobacillus stearothermophilus
80
-
1 h, 20 mM potassium phosphate, complete inactivation Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 11.5
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6 12 pH 6.0: about 75% of maximal activity, pH 12.0: about 80% of maximal activity Geobacillus stearothermophilus