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Literature summary for 2.4.2.2 extracted from
- Pyrimidine nucleoside phosphorylase from Haemophilus influenzae (1978), Methods Enzymol., 51, 432-437.
General Stability
| General Stability | Organism |
|---|---|
| repeated cycles of freezing and thawing inactivate the enzyme if phosphate is absent | Haemophilus influenzae |
| the enzyme is quite labile during the course of purification, dithiothreitol and glycerol are required for stability | Haemophilus influenzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
5-Bromouridine | - |
Haemophilus influenzae |  |
| 0.07 | - |
5-methyluridine | - |
Haemophilus influenzae | |
| 0.1 | - |
5-bromodeoxyuridine | - |
Haemophilus influenzae | |
| 0.11 | - |
thymidine | - |
Haemophilus influenzae | |
| 0.13 | - |
deoxyuridine | - |
Haemophilus influenzae | |
| 0.24 | - |
uridine | - |
Haemophilus influenzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haemophilus influenzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.5 | - |
- |
Haemophilus influenzae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in presence of phosphate, glycerol and dithiothreitol, stable for 6 months | Haemophilus influenzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-bromodeoxyuridine + phosphate | 74% of the activity with uridine | Haemophilus influenzae | 5-bromouracil + deoxyribose 1-phosphate | - |
? | |
| 5-bromouridine + phosphate | 40% of the activity with uridine | Haemophilus influenzae | 5-bromouracil + alpha-D-ribose 1-phosphate | - |
? | |
| 5-methyluridine + phosphate | 27% of the activity with uridine | Haemophilus influenzae | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
| deoxyuridine + phosphate | 12% of the activity with uridine | Haemophilus influenzae | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| thymidine + phosphate | 21% of the activity with uridine | Haemophilus influenzae | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| uracil arabinoside + phosphate | - |
Haemophilus influenzae | uracil + alpha-D-arabinose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Haemophilus influenzae | uracil + alpha-D-ribose 1-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
- |
Haemophilus influenzae |
| 7.4 | - |
ribonucleosides and uracil arabinoside | Haemophilus influenzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 7.4 | pH 6.9: optimum, pH 7.4: 75% of maximal activity | Haemophilus influenzae |
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