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Literature summary for 2.4.2.2 extracted from
- Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2 (1996), Biosci. Biotechnol. Biochem., 60, 1179-1180.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.38 | - |
uridine | - |
Haemophilus influenzae |  |
| 1.9 | - |
thymidine | - |
Haemophilus influenzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000, SDS-PAGE | Haemophilus influenzae |
| 92000 | - |
gel filtration | Haemophilus influenzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | - |
TH 6-2 | - |
| Haemophilus influenzae TH 6-2 | - |
TH 6-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haemophilus influenzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 205 | - |
- |
Haemophilus influenzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyuridine + phosphate | 147% of the activity with uridine | Haemophilus influenzae | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| deoxyuridine + phosphate | 147% of the activity with uridine | Haemophilus influenzae TH 6-2 | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| ribofuranosyl thymine + phosphate | - |
Haemophilus influenzae | thymine + alpha-D-ribose 1-phosphate | - |
? | |
| ribofuranosyl thymine + phosphate | - |
Haemophilus influenzae TH 6-2 | thymine + alpha-D-ribose 1-phosphate | - |
? | |
| thymidine + phosphate | 99% of the activity with uridine | Haemophilus influenzae | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| thymidine + phosphate | 99% of the activity with uridine | Haemophilus influenzae TH 6-2 | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Haemophilus influenzae | uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Haemophilus influenzae TH 6-2 | uracil + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46000, SDS-PAGE | Haemophilus influenzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Haemophilus influenzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable below | Haemophilus influenzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | stable | Haemophilus influenzae |
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Release 2023.1 (January 2023)
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