Protein Variants | Comment | Organism |
---|---|---|
D83G/F149S | mutation leads to monomerization, apparent melting temperature is 9.5°C lower than the wild-type value | Saccharolobus solfataricus |
I36E/M47D | mutation leads to monomerization, apparent melting temperature is 11.5°C lower than the wild-type value | Saccharolobus solfataricus |
I36E/M47D/D83G/F149S | mutation leads to monomerization, apparent melting temperature is 21.4°C lower than the wild-type value. kcat/Km for anthranilate is 14.5fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 33fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
I36E/M47D/D83G/F149S/F193S | mutation leads to monomerization, apparent melting temperature is 17.4°C lower than the wild-type value. kcat/Km for anthranilate is 52fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 31.3fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
I36E/M47D/D83G/F149S/I169T | mutation leads to monomerization, apparent melting temperature is 20.6°C lower than the wild-type value. kcat/Km for anthranilate is 8.9fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 24.3fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
I36E/M47D/D83G/F149S/L320M | mutation leads to monomerization, apparent melting temperature is 20.9°C lower than the wild-type value. kcat/Km for anthranilate is 11fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 31.3fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
I36E/M47D/D83G/N109S/F149S | mutation leads to monomerization, apparent melting temperature is 20.5°C lower than the wild-type value. kcat/Km for anthranilate is 39fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 10fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
I36E/M47D/D83G/N109S/F149S/I169T/L320M/N324I | mutation leads to monomerization, apparent melting temperature is 18.5°C lower than the wild-type value | Saccharolobus solfataricus |
I36E/M47D/T77I/D83G/F149S | mutation leads to monomerization, apparent melting temperature is 13.3°C lower than the wild-type value. kcat/Km for anthranilate is 13.3fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 10fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
I36E/M47D/T77I/D83G/F149S/F193S | mutation leads to monomerization, apparent melting temperature is 11.4°C lower than the wild-type value | Saccharolobus solfataricus |
I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M | mutation leads to monomerization, apparent melting temperature is 9.1°C lower than the wild-type value. kcat/Km for anthranilate is 85.7fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 2.7fold lower than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M | mutation leads to monomerization, apparent melting temperature is 8.7°C lower than the wild-type value. kcat/Km for anthranilate is 209fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.008 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.011 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.029 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.033 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.037 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.037 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.045 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.047 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.051 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.054 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.055 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.055 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.06 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.062 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.082 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharolobus solfataricus | catalyzes the third step in tryptophan biosynthesis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharolobus solfataricus P2 | catalyzes the third step in tryptophan biosynthesis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P50384 | - |
- |
Saccharolobus solfataricus P2 | P50384 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | catalyzes the third step in tryptophan biosynthesis | Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus P2 | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | catalyzes the third step in tryptophan biosynthesis | Saccharolobus solfataricus P2 | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sAnPRT | - |
Saccharolobus solfataricus |
SSO0890 | locus name | Saccharolobus solfataricus |
strpD | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.046 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.78 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.78 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
1.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
1.2 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
1.3 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
1.3 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
2.7 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
2.7 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
3.4 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
3.4 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
3.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
3.5 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
3.9 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
3.9 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | catalyzes the third step in tryptophan biosynthesis | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
0.84 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
13 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
23 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
24 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
24 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
25 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
56 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
72 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
72 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
76 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
92 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
330 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
360 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
460 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
540 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus |