Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni |
Crystallization (Comment) | Organism |
---|---|
in complex with ATP, L-histidine, or L-histidine/AMP, hanging drop vapor diffusion method, using 0.1M sodium acetate, 0.1 M MgCl2, 13-15% (w/v) PEG 4000, pH 5.5 (ATP), or 0.1 M BTP, 0.2 M KSCN, 13-14% (w/v) PEG 3350, pH 6.5 (His), or 0.1 M Tris, 0.1 M MgCl2, 13-15% (w/v) PEG 4000, pH 7.5 (L-His/AMP) | Campylobacter jejuni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | competitive inhibition | Campylobacter jejuni | |
L-histidine | allosterical inhibition | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Campylobacter jejuni | |
0.097 | - |
ATP | at pH 8.5 and 25°C | Campylobacter jejuni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential | Campylobacter jejuni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
184000 | - |
gel filtration | Campylobacter jejuni |
188000 | - |
SEC-coupled static light scattering | Campylobacter jejuni |
202000 | - |
calculated from amino acid sequence | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Campylobacter jejuni | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Campylobacter jejuni NCTC 11168 | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Campylobacter jejuni | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Campylobacter jejuni NCTC 11168 | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | Q9PM78 | - |
- |
Campylobacter jejuni NCTC 11168 | Q9PM78 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Talon column chromatography and Superdex 200 gel filtration | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Campylobacter jejuni | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Campylobacter jejuni NCTC 11168 | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Campylobacter jejuni | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Campylobacter jejuni NCTC 11168 | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
adenosine triphosphate phosphoribosyltransferase | - |
Campylobacter jejuni |
ATP-PRT | - |
Campylobacter jejuni |
HisGL | - |
Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Campylobacter jejuni |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-histidine | with respect to 5-phospho-alpha-D-ribose 1-diphosphate, at pH 8.5 and 25°C | Campylobacter jejuni | |
0.044 | - |
L-histidine | with respect to ATP, at pH 8.5 and 25°C | Campylobacter jejuni | |
0.356 | - |
AMP | with respect to ATP, at pH 8.5 and 25°C | Campylobacter jejuni | |
0.382 | - |
AMP | with respect to 5-phospho-alpha-D-ribose 1-diphosphate, at pH 8.5 and 25°C | Campylobacter jejuni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
ATP | at pH 8.5 and 25°C | Campylobacter jejuni | |
120 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Campylobacter jejuni |