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Literature summary for 2.4.2.17 extracted from
- The first enzyme in histidine biosynthesis: the nature of feedback inhibition by histidine (1963), J. Biol. Chem., 238, 257-268.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| L-histidine, 0.4 mM, stabilizes against heat inactivation, 0.04 mM does not stabilize against heat inactivation, at 1.33 mM and higher heat inactivation is greater than the control | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-methyl-L-histidine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 2-thiazol-DL-alanine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| adenine | competitive to ATP and 5-phospho-alpha-D-ribose 1-diphosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
| ADP | competitive to ATP, in the presence of histidine inhibition by AMP and ADP becomes positively cooperative and much more potent | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Ag+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| AMP | competitive inhibitor to 5-phospho-alpha-D-ribose 1-diphosphate, in the presence of histidine inhibition by AMP and ADP becomes positively cooperative and much more potent | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Cd2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Co2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Cu2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Hg2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| L-histidine | feed-back inhibition; reversed by Hg2+, p-hydroxymercuribenzoate, methylmercuric bromide, Ni2+; the L-configuration is essential, substitution of alpha-amino group appreciably reduces inhibition, non competitive inhibitor with respect to both substrates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Methylmercuric bromide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| N-ethylmaleimide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Ni2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| p-hydroxymercuribenzoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Zn2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.2 | - |
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Salmonella enterica subsp. enterica serovar Typhimurium | first step in histidine biosynthesis | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and DEAE-cellulose chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
description of assay method | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 1-(5-phospho-D-ribosyl)-ATP + diphosphate | - |
? | |
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | first step in histidine biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
very sensitive to heat inactivation, 0.4 mM histidine stabilizes the enzyme to inactivation by heat | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
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Release 2023.1 (January 2023)
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