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Literature summary for 2.4.2.14 extracted from

  • Chen, S.; Tomchick, D.R.; Wolle, D.; Hu, P.; Smith, J.L.; Switzer, R.L.; Zalkin, H.
    Mechanism of the synergistic end-product regulation of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase by nucleotides (1997), Biochemistry, 36, 10718-10726.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the ternary enzyme-ADP-GMP complex are grown in glass melting point capillaries by the microbath method, 20 mg/ml enzyme are incubated with 1 mM ADP, 1 mM GMP, and 5 mM MgCl2 and mixed with an equal volume of 24% polyethylene glycol 8000, 200 mM KCl, 50 mM N-(2-hydroxyethyl)piperazine-N'-3-propanesulfonic acid, pH 7.9, brown crystals emerge after 6-12 weeks Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
ADP 50% inhibition of wild-type, S283A, K305Q, R307Q and S347A mutant enzyme at 4.7 mM, 24 mM, 31 mM, 28 mM and 8.1 mM respectively Bacillus subtilis
AMP 50% inhibition of wild-type, S283A, K305Q, R307Q and S347A mutant enzyme at 0.9 mM, 6.1 mM, 2.5 mM, 2.6 mM and 1.5 mM respectively Bacillus subtilis
GMP 50% inhibition of wild-type, S283A, K305Q, R307Q and S347A mutant enzyme at 9.4 mM, 6.6 mM, 50 mM, 50 mM and 145 mM respectively Bacillus subtilis
IMP 50% inhibition of wild-type and S347A mutant enzyme at 26 mM and 41 mM respectively Bacillus subtilis
additional information strong synergistic inhibition with ADP and GMP Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P00497
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