Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.14 extracted from

  • Reynoldds, P.H.S.; Blevins, D.G.; Randall, D.D.
    5-Phosphoribosylpyrophosphate amidotransferase from soybean root nodules: kinetic and regulatory properties (1984), Arch. Biochem. Biophys., 229, 623-631.
    View publication on PubMed
Search for more literature for 2.4.2.14

Activating Compound

Activating Compound Comment Organism Structure
Mg-phosphoribosyldiphosphate requirement, phosphoribosyldiphosphate-Mg3- is the reactive molecular species of phosphoribosyldiphosphate, Km-value: 0.62 mM Glycine max

General Stability

General Stability Organism
freezing inactivates, not stabilized by 30% glycerol Glycine max
high concentrations of thiol reagents stabilize during purification Glycine max

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive vs. 5-phospho-alpha-D-ribose 1-diphosphate Glycine max
diphosphate uncompetitive vs. 5-phospho-alpha-D-ribose 1-diphosphate Glycine max
glutamate competitive vs. glutamate Glycine max
GMP
-
 Glycine max
IMP
-
 Glycine max
NH4+ competitive vs. glutamine Glycine max
XMP competitive vs. 5-phospho-alpha-D-ribose 1-diphosphate Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Glycine max
0.4
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Glycine max
16
-
 NH3
-
 Glycine max
16
-
 NH4+
-
 Glycine max
18
-
 L-glutamine
-
 Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Glycine max
Mg2+ 5-phospho-alpha-D-ribose 1-diphosphate-Mg3- is the reactive molecular species Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8000000
-
 probably an aggregation of several purine synthetic activities, gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O Glycine max enzyme of purine biosynthetic pathway, regulatory enzyme in the flow of recently fixed nitrogen from initial assimilation into amino acids via purine biosynthesis L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
 soybean, Merr. cv. Williams
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose CL-2B, glycerol gradient Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.6
-
-
 Glycine max

Storage Stability

Storage Stability Organism
4°C, 25% loss of activity per day Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O reaction at 50% the rate of aminotransferase activity Glycine max 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
 ?
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O enzyme of purine biosynthetic pathway, regulatory enzyme in the flow of recently fixed nitrogen from initial assimilation into amino acids via purine biosynthesis Glycine max L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate
-
 ?
NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O 2fold higher aminotransferase activity compared to amidotransferase activity Glycine max 5-phospho-beta-D-ribosylamine + diphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
6 10 approx. 70% of maximal activity between pH 6 and pH 10 Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.65
-
 diphosphate
-
 Glycine max
1.2
-
 XMP
-
 Glycine max
2
-
 AMP
-
 Glycine max
16
-
 NH4+
-
 Glycine max
30
-
 glutamate
-
 Glycine max