Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | no inhibition in the presence of 5-phospho-alpha-D-ribose 1-diphosphate at high concentrations | Rattus norvegicus | |
GMP | no inhibition in the presence of 5-phospho-alpha-D-ribose 1-diphosphate at high concentrations | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.24 | 1.5 | L-glutamine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
195000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
200000 | - |
native PAGE | Rattus norvegicus |
215000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Rattus norvegicus | rate-limiting enzyme of purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat treatment, acid precipitation, ammonium sulfate, ethanol, Sephadex G-150, DE-52 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.82 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Rattus norvegicus | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | rate-limiting enzyme of purine biosynthesis | Rattus norvegicus | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | broad, Tris or phosphate buffer, constant activity | Rattus norvegicus |