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Literature summary for 2.4.2.14 extracted from
- Presence, preliminary properties and partial purification of 5-phosphoribosylpyrophosphate amidotransferase from Artemia sp (1990), Biochim. Biophys. Acta, 1033, 114-117.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5',5'-P1,P4-diguanosine tetraphosphate | IC50: 0.4 mM, 2 mM, approx. 80% inhibition | Artemia sp. |  |
| ADP | 1 mM, approx. 95% inhibition | Artemia sp. | |
| AMP | 1 mM, approx. 40% inhibition | Artemia sp. | |
| GDP | 1 mM, approx. 75% inhibition | Artemia sp. | |
| GMP | 1 mM, approx. 60% inhibition | Artemia sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
5-phospho-alpha-D-ribose 1-diphosphate | cosubstrate NH3 | Artemia sp. | |
| 0.7 | - |
5-phospho-alpha-D-ribose 1-diphosphate | cosubstrate glutamine | Artemia sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Artemia sp. | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemia sp. | - |
brine shrimp, activity increases 7fold during early larval development in 2 d old Artemia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Artemia sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| nauplius | - |
Artemia sp. | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Artemia sp. | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
ir | |
| NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | lower aminotransferase activity compared to amidotransferase activity | Artemia sp. | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Artemia sp. |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
IC50: 0.4 mM, 2 mM, approx. 80% inhibition | Artemia sp. | 5',5'-P1,P4-diguanosine tetraphosphate | |
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