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Literature summary for 2.4.2.1 extracted from

  • Nannemann, D.; Kaufmann, K.; Meiler, J.; Bachmann, B.
    Design and directed evolution of a dideoxy purine nucleoside phosphorylase (2010), Protein Eng. Des. Sel., 23, 607-616.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y88A mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88C mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88D mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88E mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88F engineering of human enzyme to accept 2',3'-dideoxyinosine as substrate. Mutant displays 9-fold improvement in kcat and greater than 2-fold reduction in KM of 2',3'-dideoxyinosine Homo sapiens
Y88H mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88I mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88K mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88L mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88M mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88N mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88Q mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88R mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88S mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88T mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88V mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens
Y88W mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 Inosine wild-type, pH 7.0, 25°C Homo sapiens
0.073
-
 Inosine mutant Y88F, pH 7.0, 25°C Homo sapiens
0.074
-
 Inosine mutant Y88H, pH 7.0, 25°C Homo sapiens
0.08
-
 Inosine mutant Y88A, pH 7.0, 25°C Homo sapiens
0.094
-
 Inosine mutant Y88L, pH 7.0, 25°C Homo sapiens
0.17
-
 Inosine mutant Y88C, pH 7.0, 25°C Homo sapiens
0.345
-
 Inosine mutant Y88M, pH 7.0, 25°C Homo sapiens
0.37
-
 Inosine mutant Y88R, pH 7.0, 25°C Homo sapiens
0.391
-
 2',3'-dideoxyinosine mutant Y88L, pH 7.0, 25°C Homo sapiens
0.43
-
 Inosine mutant Y88S, pH 7.0, 25°C Homo sapiens
0.45
-
 2',3'-dideoxyinosine mutant Y88F, pH 7.0, 25°C Homo sapiens
0.5
-
 Inosine mutant Y88W, pH 7.0, 25°C Homo sapiens
0.645
-
 2',3'-dideoxyinosine mutant Y88M, pH 7.0, 25°C Homo sapiens
0.675
-
 2',3'-dideoxyinosine mutant Y88H, pH 7.0, 25°C Homo sapiens
0.81
-
 2',3'-dideoxyinosine mutant Y88C, pH 7.0, 25°C Homo sapiens
0.845
-
 Inosine mutant Y88M, pH 7.0, 25°C Homo sapiens
0.855
-
 Inosine mutant Y88Q, pH 7.0, 25°C Homo sapiens
0.86
-
 2',3'-dideoxyinosine mutant Y88Q, pH 7.0, 25°C Homo sapiens
0.98
-
 2',3'-dideoxyinosine mutant Y88W, pH 7.0, 25°C Homo sapiens
1.03
-
 2',3'-dideoxyinosine wild-type, pH 7.0, 25°C Homo sapiens
1.2
-
 Inosine mutant Y88T, pH 7.0, 25°C Homo sapiens
1.2
-
 Inosine mutant Y88V, pH 7.0, 25°C Homo sapiens
1.42
-
 Inosine mutant Y88I, pH 7.0, 25°C Homo sapiens
1.67
-
 2',3'-dideoxyinosine mutant Y88A, pH 7.0, 25°C Homo sapiens
2.6
-
 Inosine mutant Y88D, pH 7.0, 25°C Homo sapiens
4.06
-
 2',3'-dideoxyinosine mutant Y88S, pH 7.0, 25°C Homo sapiens
4.41
-
 2',3'-dideoxyinosine mutant Y88V, pH 7.0, 25°C Homo sapiens
4.67
-
 2',3'-dideoxyinosine mutant Y88I, pH 7.0, 25°C Homo sapiens
4.86
-
 Inosine mutant Y88E, pH 7.0, 25°C Homo sapiens
8.16
-
 2',3'-dideoxyinosine mutant Y88T, pH 7.0, 25°C Homo sapiens
9.53
-
 2',3'-dideoxyinosine mutant Y88M, pH 7.0, 25°C Homo sapiens
12.7
-
 2',3'-dideoxyinosine mutant Y88D, pH 7.0, 25°C Homo sapiens
14.9
-
 2',3'-dideoxyinosine mutant Y88E, pH 7.0, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00491
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxyinosine + phosphate
-
 Homo sapiens hypoxanthine + 2,3-dideoxy-alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 2',3'-dideoxyinosine mutant Y88E, pH 7.0, 25°C Homo sapiens
0.01
-
 Inosine mutant Y88R, pH 7.0, 25°C Homo sapiens
0.02
-
 2',3'-dideoxyinosine mutant Y88D, pH 7.0, 25°C Homo sapiens
0.09
-
 2',3'-dideoxyinosine mutant Y88W, pH 7.0, 25°C Homo sapiens
0.15
-
 2',3'-dideoxyinosine mutant Y88Q, pH 7.0, 25°C Homo sapiens
0.4
-
 2',3'-dideoxyinosine mutant Y88T, pH 7.0, 25°C Homo sapiens
0.5
-
 2',3'-dideoxyinosine mutant Y88I, pH 7.0, 25°C Homo sapiens
0.6
-
 2',3'-dideoxyinosine mutant Y88V, pH 7.0, 25°C Homo sapiens
0.7
-
 Inosine mutant Y88E, pH 7.0, 25°C Homo sapiens
0.8
-
 Inosine mutant Y88D, pH 7.0, 25°C Homo sapiens
0.9
-
 2',3'-dideoxyinosine wild-type, pH 7.0, 25°C Homo sapiens
1.3
-
 Inosine mutant Y88I, pH 7.0, 25°C Homo sapiens
1.4
-
 Inosine mutant Y88Q, pH 7.0, 25°C Homo sapiens
1.5
-
 2',3'-dideoxyinosine mutant Y88M, pH 7.0, 25°C Homo sapiens
2.3
-
 Inosine mutant Y88T, pH 7.0, 25°C Homo sapiens
2.4
-
 Inosine mutant Y88V, pH 7.0, 25°C Homo sapiens
2.5
-
 2',3'-dideoxyinosine mutant Y88C, pH 7.0, 25°C Homo sapiens
2.5
-
 2',3'-dideoxyinosine mutant Y88H, pH 7.0, 25°C Homo sapiens
2.7
-
 2',3'-dideoxyinosine mutant Y88S, pH 7.0, 25°C Homo sapiens
2.8
-
 2',3'-dideoxyinosine mutant Y88A, pH 7.0, 25°C Homo sapiens
5.2
-
 2',3'-dideoxyinosine mutant Y88M, pH 7.0, 25°C Homo sapiens
5.5
-
 Inosine mutant Y88C, pH 7.0, 25°C Homo sapiens
5.6
-
 Inosine mutant Y88W, pH 7.0, 25°C Homo sapiens
6.1
-
 2',3'-dideoxyinosine mutant Y88L, pH 7.0, 25°C Homo sapiens
8.6
-
 Inosine mutant Y88H, pH 7.0, 25°C Homo sapiens
8.8
-
 Inosine mutant Y88S, pH 7.0, 25°C Homo sapiens
9.7
-
 Inosine mutant Y88M, pH 7.0, 25°C Homo sapiens
10.5
-
 2',3'-dideoxyinosine mutant Y88F, pH 7.0, 25°C Homo sapiens
12.8
-
 Inosine mutant Y88M, pH 7.0, 25°C Homo sapiens
15.9
-
 Inosine mutant Y88L, pH 7.0, 25°C Homo sapiens
17.6
-
 Inosine mutant Y88A, pH 7.0, 25°C Homo sapiens
28.3
-
 Inosine mutant Y88F, pH 7.0, 25°C Homo sapiens
43.9
-
 Inosine wild-type, pH 7.0, 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00014
-
 Inosine mutant Y88E, pH 7.0, 25°C Homo sapiens
0.00029
-
 Inosine mutant Y88D, pH 7.0, 25°C Homo sapiens
0.0009
-
 2',3'-dideoxyinosine mutant Y88E, pH 7.0, 25°C Homo sapiens
0.0009
-
 Inosine mutant Y88I, pH 7.0, 25°C Homo sapiens
0.0017
-
 Inosine mutant Y88Q, pH 7.0, 25°C Homo sapiens
0.0018
-
 2',3'-dideoxyinosine mutant Y88D, pH 7.0, 25°C Homo sapiens
0.0019
-
 Inosine mutant Y88T, pH 7.0, 25°C Homo sapiens
0.0019
-
 Inosine mutant Y88V, pH 7.0, 25°C Homo sapiens
0.0114
-
 Inosine mutant Y88M, pH 7.0, 25°C Homo sapiens
0.013
-
 Inosine mutant Y88W, pH 7.0, 25°C Homo sapiens
0.0203
-
 Inosine mutant Y88S, pH 7.0, 25°C Homo sapiens
0.0321
-
 Inosine mutant Y88C, pH 7.0, 25°C Homo sapiens
0.0371
-
 Inosine mutant Y88M, pH 7.0, 25°C Homo sapiens
0.044
-
 2',3'-dideoxyinosine mutant Y88T, pH 7.0, 25°C Homo sapiens
0.091
-
 2',3'-dideoxyinosine mutant Y88W, pH 7.0, 25°C Homo sapiens
0.115
-
 2',3'-dideoxyinosine mutant Y88I, pH 7.0, 25°C Homo sapiens
0.116
-
 Inosine mutant Y88H, pH 7.0, 25°C Homo sapiens
0.144
-
 2',3'-dideoxyinosine mutant Y88V, pH 7.0, 25°C Homo sapiens
0.153
-
 2',3'-dideoxyinosine mutant Y88M, pH 7.0, 25°C Homo sapiens
0.169
-
 Inosine mutant Y88L, pH 7.0, 25°C Homo sapiens
0.177
-
 2',3'-dideoxyinosine mutant Y88Q, pH 7.0, 25°C Homo sapiens
0.219
-
 Inosine mutant Y88A, pH 7.0, 25°C Homo sapiens
0.389
-
 Inosine mutant Y88F, pH 7.0, 25°C Homo sapiens
0.652
-
 2',3'-dideoxyinosine mutant Y88S, pH 7.0, 25°C Homo sapiens
0.875
-
 2',3'-dideoxyinosine wild-type, pH 7.0, 25°C Homo sapiens
0.921
-
 Inosine wild-type, pH 7.0, 25°C Homo sapiens
1.67
-
 2',3'-dideoxyinosine mutant Y88A, pH 7.0, 25°C Homo sapiens
3.07
-
 2',3'-dideoxyinosine mutant Y88C, pH 7.0, 25°C Homo sapiens
3.67
-
 2',3'-dideoxyinosine mutant Y88H, pH 7.0, 25°C Homo sapiens
8.09
-
 2',3'-dideoxyinosine mutant Y88M, pH 7.0, 25°C Homo sapiens
15.67
-
 2',3'-dideoxyinosine mutant Y88L, pH 7.0, 25°C Homo sapiens
23.14
-
 2',3'-dideoxyinosine mutant Y88F, pH 7.0, 25°C Homo sapiens