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Literature summary for 2.4.2.1 extracted from

  • Xie, X.; Xia, J.; He, K.; Lu, L.; Xu, Q.; Chen, N.
    Low-molecular-mass purine nucleoside phosphorylase: characterization and application in enzymatic synthesis of nucleoside antiviral drugs (2011), Biotechnol. Lett., 33, 1107-1112.
    View publication on PubMed
Search for more literature for 2.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis
expression in Escherichia coli Escherichia coli
expression in Escherichia coli Pseudoalteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00031
-
 Inosine isoform of 816 amino acids, pH 7.0, 37°C Bacillus subtilis
0.00045
-
 Inosine pH 7.0, 37°C Pseudoalteromonas sp.
0.00085
-
 Inosine pH 7.0, 37°C Escherichia coli
0.012
-
 Inosine isoform of 702 amino acids, pH 7.0, 37°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-
Escherichia coli
-
-
-
Pseudoalteromonas sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + phosphate 12% of the activity with inosine Escherichia coli adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2'-deoxyadenosine + phosphate isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine Bacillus subtilis adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2'-deoxyadenosine + phosphate isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine Bacillus subtilis 168 adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2'-deoxyadenosine + phosphate 10% of the activity with inosine Pseudoalteromonas sp. 2-deoxy-alpha-D-ribose 1-phosphate + adenine
-
 ?
2'-deoxyguanosine + phosphate 20% of the activity with inosine Escherichia coli guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2'-deoxyguanosine + phosphate isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine Bacillus subtilis guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2'-deoxyguanosine + phosphate isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine Bacillus subtilis 168 guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2'-deoxyinosine + phosphate 22% of the activity with inosine Escherichia coli hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2'-deoxyinosine + phosphate isoform of 816 amino acids, 23% of the activity with inosine, isoform of 702 amino acids, 31% of the activity with inosine Bacillus subtilis hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
adenosine + phosphate 41% of the activity with inosine Escherichia coli adenine + alpha-D-ribose 1-phosphate
-
 ?
adenosine + phosphate 45% of the activity with inosine Pseudoalteromonas sp. adenine + alpha-D-ribose 1-phosphate
-
 ?
adenosine + phosphate isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine Bacillus subtilis adenine + alpha-D-ribose 1-phosphate
-
 ?
adenosine + phosphate isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine Bacillus subtilis 168 adenine + alpha-D-ribose 1-phosphate
-
 ?
deoxyinosine + phosphate 14% of the activity with inosine Pseudoalteromonas sp. hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
deoxyinosine + phosphate 19% of the activity with inosine Pseudoalteromonas sp. hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
guanosine + phosphate 56% of the activity with inosine Pseudoalteromonas sp. guanine + alpha-D-ribose 1-phosphate
-
 ?
guanosine + phosphate 66% of the activity with inosine Escherichia coli guanine + alpha-D-ribose 1-phosphate
-
 ?
guanosine + phosphate isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine Bacillus subtilis guanine + alpha-D-ribose 1-phosphate
-
 ?
guanosine + phosphate isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine Bacillus subtilis 168 guanine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Bacillus subtilis hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Pseudoalteromonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Bacillus subtilis 168 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3900
-
 Inosine isoform of 702 amino acids, pH 7.0, 37°C Bacillus subtilis
6200
-
 Inosine pH 7.0, 37°C Escherichia coli
7000
-
 Inosine pH 7.0, 37°C Pseudoalteromonas sp.
12000
-
 Inosine isoform of 816 amino acids, pH 7.0, 37°C Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
74000
-
 Inosine pH 7.0, 37°C Escherichia coli
160000
-
 Inosine pH 7.0, 37°C Pseudoalteromonas sp.
320000
-
 Inosine isoform of 702 amino acids, pH 7.0, 37°C Bacillus subtilis
3900000
-
 Inosine isoform of 816 amino acids, pH 7.0, 37°C Bacillus subtilis