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Literature summary for 2.4.2.1 extracted from
- Purine nucleoside phosphorylase (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 7, 483-514.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Escherichia coli |
| medicine | - |
Bos taurus |
| medicine | the purine nucleoside phosphorylases may play a role in chemotherapy in two ways: 1. by catalyzing the breakdown of nucleosides that contain purine analogs and by promoting the incorporation of purine and pyrimidine analogs into the nucleotides and nucleic acids of the cell by increasing the intracellular pool of ribose 1-phosphate and deoxyribose 1-phosphate | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Amino-6-methylthiopurine | - |
Homo sapiens |  |
| 6-Mercaptopurine | - |
Homo sapiens | |
| 6-methylthiopurine | - |
Homo sapiens | |
| 6-Thioxanthine | - |
Homo sapiens | |
| 7-Deazainosine | - |
Bos taurus | |
| 7-Deazainosine | - |
Escherichia coli | |
| 7-Deazainosine | - |
Homo sapiens | |
| 7-deazathioinosine | - |
Homo sapiens | |
| adenine | - |
Homo sapiens | |
| allopurinol | - |
Homo sapiens | |
| deoxyinosine | - |
Homo sapiens | |
| diphosphate | - |
Homo sapiens | |
| formycin B | - |
Bos taurus | |
| formycin B | - |
Escherichia coli | |
| formycin B | - |
Homo sapiens | |
| guanine | - |
Bos taurus | |
| guanine | - |
Escherichia coli | |
| guanine | - |
Gallus gallus | |
| guanine | - |
Homo sapiens | |
| guanine | - |
Oryctolagus cuniculus | |
| hypoxanthine | - |
Bos taurus | |
| hypoxanthine | - |
Homo sapiens | |
| hypoxanthine | - |
Oryctolagus cuniculus | |
| Inosine | - |
Bacillus subtilis | |
| Inosine | - |
Homo sapiens | |
| Inosine | - |
Rattus norvegicus | |
| Oxoallopurinol | - |
Homo sapiens | |
| phosphate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli | |
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | - |
Bos taurus | |
| 0.019 | - |
hypoxanthine | - |
Homo sapiens | |
| 0.07 | - |
6-mercaptopurine riboside | - |
Homo sapiens | |
| 0.11 | - |
Inosine | enzyme from vegetative cells | Bacillus cereus | |
| 0.14 | - |
Inosine | enzyme from spores | Bacillus cereus | |
| 0.17 | - |
Inosine | - |
Bos taurus | |
| 0.18 | - |
deoxyguanosine | - |
Bos taurus | |
| 0.18 | - |
deoxyinosine | - |
Escherichia coli | |
| 0.18 | - |
deoxyinosine | - |
Bacillus cereus | |
| 0.216 | - |
guanosine | - |
Rattus norvegicus | |
| 0.27 | - |
hypoxanthine | - |
Rattus norvegicus | |
| 0.34 | - |
adenine | - |
Rattus norvegicus | |
| 0.376 | - |
phosphate | - |
Rattus norvegicus | |
| 0.4 | - |
hypoxanthine | - |
Bos taurus | |
| 0.41 | - |
adenine | - |
Homo sapiens | |
| 0.5 | - |
ribose 1-phosphate | - |
Bos taurus | |
| 0.74 | - |
phosphate | - |
Homo sapiens | |
| 0.8 | - |
guanine | - |
Homo sapiens | |
| 1.8 | - |
arsenate | - |
Homo sapiens | |
| 5.1 | - |
phosphate | enzyme from vegetative cells | Bacillus cereus | |
| 7.2 | - |
phosphate | enzyme from spores | Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bos taurus | - |
? | - |
? | |
| additional information | salmon | in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals | ? | - |
? | |
| additional information | Leucisus rusticus | in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals | ? | - |
? | |
| additional information | Homo sapiens | cellular function seems concerned primarily with nucleoside breakdown | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas hydrophila | - |
- |
- |
| Bacillus cereus | - |
- |
- |
| Bacillus licheniformis | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Bacterium cadaveris | - |
- |
- |
| Bos taurus | - |
- |
- |
| Brevibacillus brevis | - |
- |
- |
| Canis lupus familiaris | - |
- |
- |
| Clavibacter michiganensis subsp. sepedonicus | - |
- |
- |
| Columba livia | - |
- |
- |
| Equus caballus | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Felis catus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Klebsiella aerogenes | - |
- |
- |
| Lactobacillus leichmannii | - |
- |
- |
| Leucisus rusticus | - |
- |
- |
| Macaca mulatta | - |
- |
- |
| Micrococcus luteus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Papio hamadryas | - |
- |
- |
| Pectobacterium carotovorum | - |
- |
- |
| Proteus vulgaris | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| salmon | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Enteritidis | - |
- |
- |
| Shewanella putrefaciens | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brevibacillus brevis |
- |
Homo sapiens |
- |
Bos taurus |
- |
Pectobacterium carotovorum |
- |
Lactobacillus leichmannii |
- |
Clavibacter michiganensis subsp. sepedonicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ascites | - |
Homo sapiens | - |
| bone marrow | - |
Oryctolagus cuniculus | - |
| brain | - |
Homo sapiens | - |
| brain | - |
Bos taurus | - |
| brain | - |
Oryctolagus cuniculus | - |
| cardiac muscle | - |
Homo sapiens | - |
| erythrocyte | - |
Gallus gallus | - |
| erythrocyte | - |
Homo sapiens | - |
| erythrocyte | - |
Sus scrofa | - |
| erythrocyte | - |
Oryctolagus cuniculus | - |
| erythrocyte | - |
Canis lupus familiaris | - |
| erythrocyte | - |
Felis catus | - |
| erythrocyte | - |
Macaca mulatta | - |
| erythrocyte | - |
Columba livia | - |
| erythrocyte | - |
Papio hamadryas | - |
| gastrointestinal tract | - |
Homo sapiens | - |
| heart | - |
Macaca mulatta | - |
| intestinal mucosa | - |
Macaca mulatta | - |
| kidney | - |
Oryctolagus cuniculus | - |
| kidney | - |
Macaca mulatta | - |
| leukocyte | - |
Homo sapiens | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| liver | - |
Equus caballus | - |
| liver | - |
Macaca mulatta | - |
| lung | - |
Macaca mulatta | - |
| lymph node | - |
Macaca mulatta | - |
| skeletal muscle | - |
Homo sapiens | - |
| skeletal muscle | - |
Macaca mulatta | - |
| skin | - |
Leucisus rusticus | - |
| skin fibroblast | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| spleen | - |
Bos taurus | - |
| spleen | - |
Oryctolagus cuniculus | - |
| spleen | - |
Macaca mulatta | - |
| spore | - |
Bacillus cereus | - |
| thymus | - |
Macaca mulatta | - |
| vegetative cell | - |
Bacillus cereus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-mercaptopurine riboside + phosphate | - |
Homo sapiens | alpha-D-ribose 1-phosphate + 6-mercaptopurine | - |
? | |
| adenine + deoxyribose 1-phosphate | - |
Homo sapiens | deoxyadenosine + phosphate | - |
? | |
| adenosine + phosphate | - |
Rattus norvegicus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Bacillus subtilis | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Escherichia coli | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Homo sapiens | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Rattus norvegicus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Bos taurus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Pectobacterium carotovorum | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyinosine + phosphate | - |
Bacillus subtilis | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | - |
Bos taurus | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | - |
Klebsiella aerogenes | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | - |
Pectobacterium carotovorum | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | - |
Bacillus cereus | hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Bacillus subtilis | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Escherichia coli | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Homo sapiens | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Rattus norvegicus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Bos taurus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Proteus vulgaris | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Pectobacterium carotovorum | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Gallus gallus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Bacillus subtilis | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Rattus norvegicus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Bos taurus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Bos taurus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Oryctolagus cuniculus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Oryctolagus cuniculus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Klebsiella aerogenes | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Bacillus cereus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Proteus vulgaris | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Pectobacterium carotovorum | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| additional information | - |
Bos taurus | ? | - |
? | |
| additional information | in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals | salmon | ? | - |
? | |
| additional information | in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals | Leucisus rusticus | ? | - |
? | |
| additional information | cellular function seems concerned primarily with nucleoside breakdown | Homo sapiens | ? | - |
? | |
| xanthosine + arsenate | - |
Homo sapiens | xanthine + alpha-D-ribose 1-arsenate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli | |
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | - |
Bos taurus | |
| additional information | - |
additional information | - |
Bacillus cereus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
hypoxanthine | - |
Homo sapiens | |
| 0.073 | - |
6-Mercaptopurine | - |
Homo sapiens | |
| 0.13 | - |
6-methylthiopurine | - |
Homo sapiens | |
| 0.26 | - |
Inosine | - |
Homo sapiens | |
| 0.3 | - |
2-Amino-6-methylthiopurine | - |
Homo sapiens | |
| 0.33 | - |
diphosphate | - |
Homo sapiens | |
| 0.33 | - |
7-Deazainosine | - |
Homo sapiens | |
| 0.5 | - |
formycin B | - |
Homo sapiens | |
| 0.81 | - |
Oxoallopurinol | - |
Homo sapiens | |
| 0.97 | - |
allopurinol | - |
Homo sapiens | |
| 1.1 | - |
6-Thioxanthine | - |
Homo sapiens | |
| 1.5 | - |
deoxyinosine | - |
Homo sapiens | |
| 4.4 | - |
phosphate | with ribose 1-phosphate as variable substrate and guanine as fixed substrate | Homo sapiens | |
| 6.6 | - |
adenine | - |
Homo sapiens | |
| 26 | - |
phosphate | with guanine as variable substrate and ribose 1-phosphate as fixed substrate | Homo sapiens | |
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