Cloned (Comment) | Organism |
---|---|
gene dpy-19, recombinant expression in Drosophila melanogaster S2 cells, coexpression with receptor proteins UNC-5 or MIG-21 | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Caenorhabditis elegans | 5783 | - |
membrane | transmembrane protein, membrane topology prediction and spacing between transmembrane domains of DPY-19, overview | Caenorhabditis elegans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl D-mannosyl phosphate + MIG-21 protein | Caenorhabditis elegans | C-mannosylation at a tryptophan residue | dolichyl phosphate + C-mannosylated MIG-21 protein | - |
? | |
dolichyl D-mannosyl phosphate + UNC-5 protein | Caenorhabditis elegans | C-mannosylation at a tryptophan residue | dolichyl phosphate + C-mannosylated UNC-5 protein | - |
? | |
additional information | Caenorhabditis elegans | cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | P34413 | gene dpy-19 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl D-mannosyl phosphate + MIG-21 protein | C-mannosylation at a tryptophan residue | Caenorhabditis elegans | dolichyl phosphate + C-mannosylated MIG-21 protein | - |
? | |
dolichyl D-mannosyl phosphate + UNC-5 protein | C-mannosylation at a tryptophan residue | Caenorhabditis elegans | dolichyl phosphate + C-mannosylated UNC-5 protein | - |
? | |
dolichyl D-mannosyl phosphate + WAKW | in vitro assay using radiolabeled donor substrate and a synthetic acceptor peptide WAKW, which forms the minimal acceptor substrate | Caenorhabditis elegans | dolichyl phosphate + ? | - |
? | |
additional information | cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19 | Caenorhabditis elegans | ? | - |
? | |
additional information | C-mannosylation of tryptophan residues is an endoplasmic reticulum localized, DPY-19 enzyme-catalyzed reaction using the lipid-linked glycoside dolichol-phosphate-mannose as the donor substrate | Caenorhabditis elegans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C-mannosyltransferase | - |
Caenorhabditis elegans |
DPY-19 | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Caenorhabditis elegans |
General Information | Comment | Organism |
---|---|---|
evolution | DPY-19 exhibits topological and sequential homology to the N-glycan oligosaccharyltransferase, highlighting an evolutionary link between N- and C-glycosylation. The enzyme is related to the OST family involved in N-glycosylation | Caenorhabditis elegans |
malfunction | identical Q neuroblast migration phenotypes of dpy-19 and mig-21 mutants | Caenorhabditis elegans |
additional information | among the different types of protein glycosylation, C-mannosylation of tryptophan residues stands out because of the unique linkage formed between sugar and protein. Instead of the typical O- or N-glycosidic linkage, a C-C bond is used for attachment of a single mannose. C-mannose is characteristically found in thrombospondin type 1 repeats and in the WSXWS motif of type I cytokine receptors | Caenorhabditis elegans |
physiological function | Caenorhabditis elegans surface receptors MIG-21 and UNC-5 are acceptor substrates of DPY-19, and C-mannosylation is essential for the secretion of soluble MIG-21 | Caenorhabditis elegans |