Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Vanillyl alcohol | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.09 | - |
maltose | synthesis of vanillyl alcohol alpha-D-isomaltoside, pH 6.6, 37°C | Saccharomyces cerevisiae | |
12.96 | - |
Vanillyl alcohol | pH 6.6, 37°C | Saccharomyces cerevisiae | |
26.24 | - |
vanillyl alcohol alpha-D-glucoside | pH 6.6, 37°C | Saccharomyces cerevisiae | |
46 | - |
maltose | synthesis of vanillyl alcohol alpha-glucoside, pH 6.6, 37°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
x * 63000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53341 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
vanillyl alcohol + maltose = 4-hydroxy-3-methoxybenzyl alpha-D-glucopyranoside + D-glucose | ping-pong bi-bi mechanism | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme additionally catalyses the reaction of alpha-glucosidase/maltase, EC 3.2.1.20. The Km value of the enzyme for maltose in the synthetic reaction to vanillyl alcohol alpha-glucoside is about two times smaller than in maltose hydrolysis | Saccharomyces cerevisiae | ? | - |
? | |
vanillyl alcohol + maltose | - |
Saccharomyces cerevisiae | vanillyl alcohol alpha-D-glucoside + D-glucose | - |
? | |
vanillyl alcohol alpha-D-glucoside + maltose | - |
Saccharomyces cerevisiae | vanillyl alcohol alpha-D-isomaltoside + D-glucose | at low vanillyl alcohol concentrations up to 50 mM, or if the reaction is prolonged to 3 h at higher concentrations of vanillyl alcohol, additional transglucosylation product vanillyl alcohol alpha-isomaltoside is detected. The transglucosidation takes place at the C6 hydroxyl group of vanillyl alcohol alpha-D-glucoside | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
maltase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
8.94 | - |
Vanillyl alcohol | pH 6.6, 37°C | Saccharomyces cerevisiae |