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Literature summary for 2.4.1.B65 extracted from
- Specificity of maltase to maltose in three different directions of reaction: hydrolytic, vanillyl alcohol glucoside and vanillyl alcohol isomaltoside synthesis (2012), Biotechnol. Prog., 28, 1450-1456.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Vanillyl alcohol | - |
Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.09 | - |
maltose | synthesis of vanillyl alcohol alpha-D-isomaltoside, pH 6.6, 37°C | Saccharomyces cerevisiae | |
| 12.96 | - |
Vanillyl alcohol | pH 6.6, 37°C | Saccharomyces cerevisiae | |
| 26.24 | - |
vanillyl alcohol alpha-D-glucoside | pH 6.6, 37°C | Saccharomyces cerevisiae | |
| 46 | - |
maltose | synthesis of vanillyl alcohol alpha-glucoside, pH 6.6, 37°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
x * 63000, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P53341 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| vanillyl alcohol + maltose = 4-hydroxy-3-methoxybenzyl alpha-D-glucopyranoside + D-glucose | ping-pong bi-bi mechanism | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme additionally catalyses the reaction of alpha-glucosidase/maltase, EC 3.2.1.20. The Km value of the enzyme for maltose in the synthetic reaction to vanillyl alcohol alpha-glucoside is about two times smaller than in maltose hydrolysis | Saccharomyces cerevisiae | ? | - |
? | |
| vanillyl alcohol + maltose | - |
Saccharomyces cerevisiae | vanillyl alcohol alpha-D-glucoside + D-glucose | - |
? | |
| vanillyl alcohol alpha-D-glucoside + maltose | - |
Saccharomyces cerevisiae | vanillyl alcohol alpha-D-isomaltoside + D-glucose | at low vanillyl alcohol concentrations up to 50 mM, or if the reaction is prolonged to 3 h at higher concentrations of vanillyl alcohol, additional transglucosylation product vanillyl alcohol alpha-isomaltoside is detected. The transglucosidation takes place at the C6 hydroxyl group of vanillyl alcohol alpha-D-glucoside | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| maltase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Saccharomyces cerevisiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.94 | - |
Vanillyl alcohol | pH 6.6, 37°C | Saccharomyces cerevisiae | |
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