Cloned (Comment) | Organism |
---|---|
expression of catalytic fragment, residues 1-546 | Paeniclostridium sordellii |
expression of catalytic fragment, residues 1-551 | Clostridium novyi |
Crystallization (Comment) | Organism |
---|---|
catalytic fragment, residues 1-546, to 2.3 A resolution. Geometry suggests that the reaction runs as a circular electron transfer in a six-membered ring, which involves the deprotonation of the nucleophile by the beta-phosphoryl group of the donor substrate UDP-glucose | Paeniclostridium sordellii |
catalytic fragment, residues 1-551 to 2.85 A resolution. Geometry suggests that the reaction runs as a circular electron transfer in a six-membered ring, which involves the deprotonation of the nucleophile by the beta-phosphoryl group of the donor substrate UDP-glucose | Clostridium novyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63992 | - |
x * 63992, catalytic fragment, residues 1-546 | Paeniclostridium sordellii |
63992 | - |
x * 63992, catalytic fragment, residues 1-551 | Clostridium novyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium novyi | Q46149 | - |
- |
Paeniclostridium sordellii | Q46342 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a circular electron transfer reaction is suggested tha does not directly involve any residues from the toxin. The transfer starts with the split of the glycosidic bond leading to the most stable transient state. The split increases the pK of the phosphoryl oxygen atom, facilitating deprotonation of the accepor, and provides space for the nucleophilic attack | Clostridium novyi | ? | - |
? | |
additional information | a circular electron transfer reaction is suggested tha does not directly involve any residues from the toxin. The transfer starts with the split of the glycosidic bond leading to the most stable transient state. The split increases the pK of the phosphoryl oxygen atom, facilitating deprotonation of the accepor, and provides space for the nucleophilic attack | Paeniclostridium sordellii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63992, catalytic fragment, residues 1-546 | Paeniclostridium sordellii |
? | x * 63992, catalytic fragment, residues 1-551 | Clostridium novyi |