Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.B62 extracted from

  • Faust, C.; Ye, B.; Song, K.
    The enzymatic domain of Clostridium difficile toxin A is located within its N-terminal region (1998), Biochem. Biophys. Res. Commun., 251, 100-105.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridioides difficile

Protein Variants

Protein Variants Comment Organism
additional information construction of truncation mutants. The enzymatic domain for binding UDP-glucose, for catalytically transferring glucose to Rho A and for recognizing the interaction interface of Rho A resides in the first N-terminal 659 amino acids of toxin A Clostridioides difficile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
308000
-
x * 308000, calculated Clostridioides difficile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridioides difficile enzyme glucosylates and inactivates small GTPases of the Rho or Ras families, culminating in cytotoxicity ?
-
?
UDP-alpha-D-glucose + RhoA Clostridioides difficile
-
UDP + D-glucosyl-RhoA
-
?

Organism

Organism UniProt Comment Textmining
Clostridioides difficile P16154
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme glucosylates and inactivates small GTPases of the Rho or Ras families, culminating in cytotoxicity Clostridioides difficile ?
-
?
UDP-alpha-D-glucose + RhoA
-
Clostridioides difficile UDP + D-glucosyl-RhoA
-
?

Subunits

Subunits Comment Organism
? x * 308000, calculated Clostridioides difficile

Synonyms

Synonyms Comment Organism
toxA
-
Clostridioides difficile
toxin A
-
Clostridioides difficile

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Clostridioides difficile

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Clostridioides difficile