Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridioides difficile |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncation mutants. The enzymatic domain for binding UDP-glucose, for catalytically transferring glucose to Rho A and for recognizing the interaction interface of Rho A resides in the first N-terminal 659 amino acids of toxin A | Clostridioides difficile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
308000 | - |
x * 308000, calculated | Clostridioides difficile |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridioides difficile | enzyme glucosylates and inactivates small GTPases of the Rho or Ras families, culminating in cytotoxicity | ? | - |
? | |
UDP-alpha-D-glucose + RhoA | Clostridioides difficile | - |
UDP + D-glucosyl-RhoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | P16154 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme glucosylates and inactivates small GTPases of the Rho or Ras families, culminating in cytotoxicity | Clostridioides difficile | ? | - |
? | |
UDP-alpha-D-glucose + RhoA | - |
Clostridioides difficile | UDP + D-glucosyl-RhoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 308000, calculated | Clostridioides difficile |
Synonyms | Comment | Organism |
---|---|---|
toxA | - |
Clostridioides difficile |
toxin A | - |
Clostridioides difficile |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Clostridioides difficile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Clostridioides difficile |