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Literature summary for 2.4.1.92 extracted from

  • Yanagisawa, K.; Taniguchi, N.; Makita, A.
    Purification and properties of GM2 synthase, UDP-N-acetylgalactosamine: GM3 beta-N-acetylgalactosaminyltransferase from rat liver (1987), Biochim. Biophys. Acta, 919, 213-220.
    View publication on PubMed
Search for more literature for 2.4.1.92

General Stability

General Stability Organism
detergents stabilize Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Chelex-100
-
 Rattus norvegicus
EDTA
-
 Rattus norvegicus
UDPgalactose
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 UDP-N-acetyl-D-galactosamine
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not Rattus norvegicus
Co2+ not Rattus norvegicus
Cu2+ activation, can replace Ni2+ to some extent Rattus norvegicus
Fe2+
-
 Rattus norvegicus
Mg2+ not Rattus norvegicus
Mn2+ can replace Ni2+ to some extent Rattus norvegicus
additional information no activation by Zn2+ Rattus norvegicus
Ni2+ requirement, most active, 10-20 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 2 * 64000, SDS-PAGE Rattus norvegicus
120000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Triton X-100 solubilized, affinity chromatography on GM3-acid Sepharose Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000149
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, stable in 20% v/v glycerol, 1% v/v Triton X-100 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: blood type H glycoprotein, deglucosylated bovine submaxillary mucin, various ceramides Rattus norvegicus ?
-
 ?
UDP-N-acetyl-D-galactosamine + (N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide 73%-78% as effective as GM3(N-glycolylneuraminic acid) Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide i.e. GM2(N-acetylneuraminic acid)-ganglioside ?
UDP-N-acetyl-D-galactosamine + GM3(N-acetylneuraminic acid)-ganglioside
-
 Rattus norvegicus UDP + GM2(N-acetylneuraminic acid)-ganglioside
-
 ?
UDP-N-acetyl-D-galactosamine + GM3(N-glycolylneuraminic acid)-ganglioside best substrate Rattus norvegicus UDP + GM2(N-glycolylneuraminic acid)-ganglioside
-
 ?
UDP-N-acetyl-D-galactosamine + N-acetylneuraminelactose i.e. sialyllactose Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-N-acetylneuraminelactose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 6.9
-
 Rattus norvegicus