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Literature summary for 2.4.1.91 extracted from

  • Ishikura, N.; Mato, M.
    Partial purification and some properties of flavonol 3-O-glycosyltransferases from seedlings of Vigna mungo, with special reference to the formation of kaempferol 3-O-galactoside and 3-O-glucoside (1993), Plant Cell Physiol., 34, 329-335.
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 14 mM, stimulates Vigna mungo
2-mercaptoethanol 14 mM, activation to 157% Vigna mungo
dithioerythritol 10 mM, activation to 215% Vigna mungo
EDTA 1 mM, activation to 173% Vigna mungo

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 10 mM, 17% inhibition Vigna mungo
CoCl2 1 mM, 35% inhibition Vigna mungo
Cu2+ 1 mM CuCl2, 96% inhibition Vigna mungo
iodoacetate
-
 Vigna mungo
KCl 10 mM, 17% inhibition Vigna mungo
Mg2+ 1 mM MgCl2, 18% inhibition Vigna mungo
Mn2+ 10 mM MnCl2, 35% inhibition Vigna mungo
NEM
-
 Vigna mungo
PCMB
-
 Vigna mungo
Phenylmercuriacetate
-
 Vigna mungo
Zn2+ 1 mM ZnCl2, 96% inhibition Vigna mungo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00067
-
 kaempferol
-
 Vigna mungo
0.69
-
 kaempferol
-
 Vigna mungo
1.67
-
 UDP-D-glucose
-
 Vigna mungo

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 gel filtration Vigna mungo

Organism

Organism UniProt Comment Textmining
Vigna mungo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vigna mungo
partial Vigna mungo

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Vigna mungo
-

Storage Stability

Storage Stability Organism
-20°C, 20 mM imidazole-HCl, pH 8.0, 14 mM 2-mercaptoethanol, 10% glycerol, 35% loss of activity after 2 days, 61% loss of activity after 1 week Vigna mungo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + isorhamnetin
-
 Vigna mungo UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + isorhamnetin 108% of the activity with kaempferol Vigna mungo UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + kaempferol 7-O-glucoside 10% of the activity with kaempferol Vigna mungo UDP + kaempferol 3,7-O-diglucoside
-
 ?
UDP-alpha-D-glucose + quercetin 7-O-beta-D-glucoside 12% of the activity with kaempferol Vigna mungo UDP + quercetin 3,7-O-di-beta-D-glucoside
-
 ?
UDPglucose + kaempferol
-
 Vigna mungo UDP + kaempferol 3-O-glucoside
-
 ?
UDPglucose + kaempferol 5,7,4'-trimethyl ether 9% of the activity with kaempferol Vigna mungo UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
-
 ?
UDPglucose + kaempferol-4'-O-methylether 42% of the activity with kaempferol Vigna mungo UDP + ?
-
 ?
UDPglucose + myricetin 20% of the activity with kaempferol Vigna mungo UDP + myrecetin 3-O-glucoside
-
 ?
UDPglucose + quercetin
-
 Vigna mungo UDP + quercetin 3-O-glucoside
-
 ?
UDPglucose + quercetin 95% of the activity with quercetin Vigna mungo UDP + quercetin 3-O-glucoside
-
 ?
UDPglucose + quercetin 5-methyl ether 7% of the activity with kaempferol Vigna mungo UDP + quercetin 5-methyl ether 3-O-glucoside
-
 ?
UDPglucose + rhamnetin 98% of the activity with kaempferol Vigna mungo UDP + rhamnetin 3-O-glucoside
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in histidine-HCl buffer Vigna mungo
8
-
 in imidazole-HCl buffer Vigna mungo