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Literature summary for 2.4.1.9 extracted from

  • Pena-Cardena, A.; Rodriguez-Alegria, M.E.; Olvera, C.; Munguia, A.L.
    Synthesis of fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreum (2015), BMC Biotechnol., 15, 2-2.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged full-length wild-type enzyme and truncated mutant enzyme in Escherichia coli strain BL21 Leuconostoc citreum

Protein Variants

Protein Variants Comment Organism
additional information construction of the truncated enzyme mutant IslA4 that can synthesize high molecular weight inulin from sucrose, with a residual sucrose hydrolytic activity, and a product specificity similar to the multidomain wild-type enzyme. High sucrose concentrations shift the specificity of the reaction towards fructooligosaccharides (FOS) synthesis, which almost eliminates inulin synthesis and leads to a considerable reduction in sucrose hydrolysis. Reactions with low IslA4 activity and a high sucrose activity allow for high levels of FOS synthesis, where 70% sucrose is used for transfer reactions, with 65% corresponding to transfructosylation for the synthesis of FOS, quantitative product analysis, overview Leuconostoc citreum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Leuconostoc citreum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leuconostoc citreum synthesis of 1-kestose and nystose ?
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Leuconostoc citreum
-
 glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged full-length wild-type enzyme and truncated mutant enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and ultrafiltration Leuconostoc citreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information synthesis of 1-kestose and nystose Leuconostoc citreum ?
-
 ?
additional information the truncated enzyme mutant IslA4 can synthesize high molecular weight inulin from sucrose, with a residual sucrose hydrolytic activity, and a product specificity similar to the multidomain wild-type enzyme. High sucrose concentrations shift the specificity of the reaction towards fructooligosaccharides (FOS) synthesis, which almost eliminates inulin synthesis and leads to a considerable reduction in sucrose hydrolysis. Reactions with low IslA4 activity and a high sucrose activity allow for high levels of FOS synthesis, where 70% sucrose is used for transfer reactions, with 65% corresponding to transfructosylation for the synthesis of FOS, quantitative product analysis, overview Leuconostoc citreum ?
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Leuconostoc citreum glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?

Synonyms

Synonyms Comment Organism
IslA
-
 Leuconostoc citreum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Leuconostoc citreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Leuconostoc citreum

General Information

General Information Comment Organism
additional information inulosucrase IslA is a multidomain fructosyltransferase Leuconostoc citreum