Cloned (Comment) | Organism |
---|---|
gene inuGB, recombinant expression of His6-tagged truncated enzyme mutant lacking the cell-anchoring motif in Escherichia coli strain DH10B | Lactobacillus gasseri |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme mutant lacking the cell-anchoring motif | Lactobacillus gasseri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus gasseri | D3WYV9 | gene inuGB | - |
Lactobacillus gasseri DSM 20604 | D3WYV9 | gene inuGB | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged truncated enzyme mutant from Escherichia coli strain DH10B by nickel affinity chromatography | Lactobacillus gasseri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.5 | - |
purified recombinant truncated His-tagged enzyme, pH 5.2, 55°C | Lactobacillus gasseri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview | Lactobacillus gasseri | ? | - |
? | |
additional information | enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview | Lactobacillus gasseri DSM 20604 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Lactobacillus gasseri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
recombinant enzyme | Lactobacillus gasseri |