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Literature summary for 2.4.1.9 extracted from

  • van Hijum, S.A.; van der Maarel, M.J.; Dijkhuizen, L.
    Kinetic properties of an inulosucrase from Lactobacillus reuteri 121 (2003), FEBS Lett., 534, 207-210.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of Myc/His-tagged enzyme in Escherichia coli Limosilactobacillus reuteri

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 98% inhibition at 1 mM Limosilactobacillus reuteri
EDTA complete inhibition at 1 mM Limosilactobacillus reuteri
Fe2+ 54% inhibition at 1 mM Limosilactobacillus reuteri
Fe3+ 72% inhibition at 1 mM Limosilactobacillus reuteri
Hg2+ 97% inhibition at 1 mM Limosilactobacillus reuteri
Zn2+ 91% inhibition at 1 mM Limosilactobacillus reuteri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics at 20°C and 50°C assay temperature Limosilactobacillus reuteri

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates by 30% at 1 mM Limosilactobacillus reuteri
additional information no effect by Mg2+, K+, and Na+ at 1 mM Limosilactobacillus reuteri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (2,1-beta-D-fructosyl)n Limosilactobacillus reuteri
-
D-glucose + (2,1-beta-D-fructosyl)n+1
-
?
sucrose + (2,1-beta-D-fructosyl)n Limosilactobacillus reuteri 121
-
D-glucose + (2,1-beta-D-fructosyl)n+1
-
?

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri Q8GP32
-
-
Limosilactobacillus reuteri 121 Q8GP32
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Myc/His-tagged enzyme from Escherichia coli Limosilactobacillus reuteri

Storage Stability

Storage Stability Organism
-20°C, purified recombinant undiluted enzyme, 10% glycerol, completely stable for at least several months Limosilactobacillus reuteri
4°C, purified recombinant enzyme, undiluted stable for several days, 10times diluted stable for more than a month Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,1-beta-D-fructosyl)n transferase activity Limosilactobacillus reuteri alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 i.e. inulin ?
sucrose + (2,1-beta-D-fructosyl)n transferase activity Limosilactobacillus reuteri 121 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 i.e. inulin ?
sucrose + (2,1-beta-D-fructosyl)n
-
Limosilactobacillus reuteri D-glucose + (2,1-beta-D-fructosyl)n+1
-
?
sucrose + (2,1-beta-D-fructosyl)n
-
Limosilactobacillus reuteri 121 D-glucose + (2,1-beta-D-fructosyl)n+1
-
?
sucrose + H2O hydrolytic activity Limosilactobacillus reuteri alpha-D-glucose + beta-D-fructose
-
?
sucrose + H2O hydrolytic activity Limosilactobacillus reuteri 121 alpha-D-glucose + beta-D-fructose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Limosilactobacillus reuteri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 transferase activity: 20% activity at 20°C, 90% activity at 60°C, rapid decrease in activity above 60°C, inactive at 70°C Limosilactobacillus reuteri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
sucrose recombinant enzyme, hydrolytic reaction, pH 5.4, 20°C Limosilactobacillus reuteri
43.6
-
sucrose recombinant enzyme, transferase reaction, pH 5.4, 20°C Limosilactobacillus reuteri
88.9
-
sucrose recombinant enzyme, hydrolytic reaction, pH 5.4, 50°C Limosilactobacillus reuteri
504
-
sucrose recombinant enzyme, transferase reaction, pH 5.4, 50°C Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
Limosilactobacillus reuteri

pH Range

pH Minimum pH Maximum Comment Organism
3.7 6.5 50% activity at pH 3.7 an pH 6.5 Limosilactobacillus reuteri