Cloned (Comment) | Organism |
---|---|
expression of Myc/His-tagged enzyme in Escherichia coli | Limosilactobacillus reuteri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 98% inhibition at 1 mM | Limosilactobacillus reuteri | |
EDTA | complete inhibition at 1 mM | Limosilactobacillus reuteri | |
Fe2+ | 54% inhibition at 1 mM | Limosilactobacillus reuteri | |
Fe3+ | 72% inhibition at 1 mM | Limosilactobacillus reuteri | |
Hg2+ | 97% inhibition at 1 mM | Limosilactobacillus reuteri | |
Zn2+ | 91% inhibition at 1 mM | Limosilactobacillus reuteri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics at 20°C and 50°C assay temperature | Limosilactobacillus reuteri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates by 30% at 1 mM | Limosilactobacillus reuteri | |
additional information | no effect by Mg2+, K+, and Na+ at 1 mM | Limosilactobacillus reuteri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,1-beta-D-fructosyl)n | Limosilactobacillus reuteri | - |
D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,1-beta-D-fructosyl)n | Limosilactobacillus reuteri 121 | - |
D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | Q8GP32 | - |
- |
Limosilactobacillus reuteri 121 | Q8GP32 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant Myc/His-tagged enzyme from Escherichia coli | Limosilactobacillus reuteri |
Storage Stability | Organism |
---|---|
-20°C, purified recombinant undiluted enzyme, 10% glycerol, completely stable for at least several months | Limosilactobacillus reuteri |
4°C, purified recombinant enzyme, undiluted stable for several days, 10times diluted stable for more than a month | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Limosilactobacillus reuteri | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin | ? | |
sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Limosilactobacillus reuteri 121 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin | ? | |
sucrose + (2,1-beta-D-fructosyl)n | - |
Limosilactobacillus reuteri | D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,1-beta-D-fructosyl)n | - |
Limosilactobacillus reuteri 121 | D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + H2O | hydrolytic activity | Limosilactobacillus reuteri | alpha-D-glucose + beta-D-fructose | - |
? | |
sucrose + H2O | hydrolytic activity | Limosilactobacillus reuteri 121 | alpha-D-glucose + beta-D-fructose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Limosilactobacillus reuteri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | transferase activity: 20% activity at 20°C, 90% activity at 60°C, rapid decrease in activity above 60°C, inactive at 70°C | Limosilactobacillus reuteri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
sucrose | recombinant enzyme, hydrolytic reaction, pH 5.4, 20°C | Limosilactobacillus reuteri | |
43.6 | - |
sucrose | recombinant enzyme, transferase reaction, pH 5.4, 20°C | Limosilactobacillus reuteri | |
88.9 | - |
sucrose | recombinant enzyme, hydrolytic reaction, pH 5.4, 50°C | Limosilactobacillus reuteri | |
504 | - |
sucrose | recombinant enzyme, transferase reaction, pH 5.4, 50°C | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Limosilactobacillus reuteri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.7 | 6.5 | 50% activity at pH 3.7 an pH 6.5 | Limosilactobacillus reuteri |