Cloned (Comment) | Organism |
---|---|
cloned from cDNA library by PCR, expression, transcription and processing, of wild-type in COS-1 cells with and without HA-tag, but no detectable enzyme activity in the transfected cells by recombinant enzyme, DNA and amino acid sequence determination and analysis | Homo sapiens |
expression in COS-1 cells with and without HA-tag | Canis lupus familiaris |
expression of wild-type in T42 human bladder and Vero monkey kidney epithelial cells, enables the cells to bind a canine Escherichia coli uropathogen, which they cannot bind without transfection | Canis lupus familiaris |
Protein Variants | Comment | Organism |
---|---|---|
additional information | chimeric enzyme constructs of N-terminal canine sequence fused in frame with the C-terminal catalytic region of the human sequence, i.e. dog:human construct, and vice versa, i.e. human:dog construct, expression in COS-1 cells, activity is shown only by the human:dog construct | Canis lupus familiaris |
additional information | chimeric enzyme constructs of N-terminal canine sequence fused in frame with the C-terminal catalytic region of the human sequence, i.e. dog:human construct, and vice versa, i.e. human:dog construct, expression in COS-1 cells, activity is shown only by the human:dog construct | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Canis lupus familiaris | 16020 | - |
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment and comparison, 83% identity | Canis lupus familiaris |
additional information | - |
amino acid sequence alignment and comparison, 83% identity | Homo sapiens |
additional information | - |
genomic organisation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | variability in glycolipid synthesis between species is an important determinant of microbial tropism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Homo sapiens | - |
cosmid sequence from chromosome 9q34 | - |
Homo sapiens | Q8N5D6 | immunoreactive sequence homologue to FS sequences, but without FS activity due to modification of catalytic domain during evolution | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme mRNA is detectable in all tissues | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | variability in glycolipid synthesis between species is an important determinant of microbial tropism | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | no activity | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Forssmann glycolipid synthetase | - |
Canis lupus familiaris |
Forssmann glycolipid synthetase | - |
Homo sapiens |
FS | - |
Canis lupus familiaris |
FS | - |
Homo sapiens |