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Literature summary for 2.4.1.87 extracted from

  • Stults, C.L.M.; Macher, B.A.; Bhatti, R.; Srivastava, O.P.; Hindsgaul, O.
    Characterization of the substrate specificity of alpha1,3-galactosyltransferase utilizing modified N-acetyllactosamine disaccharides (1999), Glycobiology, 9, 661-668.
    View publication on PubMed
Search for more literature for 2.4.1.87

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activates Mus musculus

Application

Application Comment Organism
synthesis useful tool for production of a range of compounds to further investigate the binding site of anti-Gal and other proteins with Galalpha(1-3)Gal binding sites Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate
-
 Mus musculus
EDTA
-
 Mus musculus
Galbeta(1-3)GlcNAc-C8 strong Mus musculus
octyl 3-amino-3-deoxy-3-N-(2-diazo-3,3,3-trifluoropropionyl-beta-D-galactopyranosyl-(1,4)-2-acetamido-2-deoxy-beta-D-glucopyranoside) i.e. DTFP-LacNAc-C8 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values for substituted LacNAc-saccharides containing Galalpha(1-3)Gal epitope Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight activation Mus musculus
Mg2+ slight activation Mus musculus
Mn2+ pronounced requirement Mus musculus
Zn2+ slight activation Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 scale up, recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + substituted LacNAc-saccharides containing Galalpha(1-3)Gal epitope acceptor substrate substrate specificity, overview Mus musculus ?
-
 ?
UDP-galactose + substituted LacNAc-saccharides containing Galalpha(1-3)Gal epitope strictly specific for UDP-galactose Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-1,3-galactosyltransferase
-
 Mus musculus
alpha1,3GT
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.27
-
 Galbeta(1-3)GlcNAc-C8
-
 Mus musculus
0.3
-
 octyl 3-amino-3-deoxy-3-N-(2-diazo-3,3,3-trifluoropropionyl-beta-D-galactopyranosyl-(1,4)-2-acetamido-2-deoxy-beta-D-glucopyranoside)
-
 Mus musculus