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Literature summary for 2.4.1.87 extracted from
- Enzymatic synthesis of a blood group B-related pentaglycosylceramide by an alpha-galactosyltransferase from rabbit bone marrow (1973), J. Biol. Chem., 248, 1700-1706.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Oryctolagus cuniculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
UDP-galactose | - |
Oryctolagus cuniculus | |
| 1.67 | - |
galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | - |
Oryctolagus cuniculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 10-20 mM, required | Oryctolagus cuniculus | |
| Mn2+ | can only poorly be substituted by Co2+, Cd2+, Zn2+, Ni2+ | Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | Oryctolagus cuniculus | biosynthesis of blood group B specific pentaglycosylceramide | UDP + O-alpha-galactosyl-1,3-galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | - |
? | |
| UDP-galactose + galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | Oryctolagus cuniculus | biosynthesis of blood group B specific pentaglycosylceramide | UDP + O-alpha-galactosyl-1,4-galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| different detergents for solubilization, best with Triton CF-54 | Oryctolagus cuniculus |
| partial | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone marrow | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | specific for UDP-galactose as donor substrate | Oryctolagus cuniculus | UDP + O-alpha-galactosyl-1,3-galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide + O-alpha-galactosyl-1,4-galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | - |
? | |
| UDP-galactose + galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | biosynthesis of blood group B specific pentaglycosylceramide | Oryctolagus cuniculus | UDP + O-alpha-galactosyl-1,3-galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | - |
? | |
| UDP-galactose + galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | biosynthesis of blood group B specific pentaglycosylceramide | Oryctolagus cuniculus | UDP + O-alpha-galactosyl-1,4-galactosyl-1,3-N-acetylglucosaminyl-1,3-galactosyl-1,4-glucosyl-1,1-ceramide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Oryctolagus cuniculus |
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