Literature summary for 2.4.1.85 extracted from

Thorsoe, K.S.; Bak, S.; Olsen, C.E.; Imberty, A.; Breton, C.; Moller, B.L.
Determination of catalytic key amino acids and UDP sugar donor specificity of the cyanohydrin glycosyltransferase UGT85B1 from Sorghum bicolor. Molecular modeling substantiated by site-specific mutagenesis and biochemical analyses (2005), Plant Physiol., 139, 664-673.

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Cloned(Commentary)

Cloned (Comment)	Organism
mutant enzymes, expression in Escherichia coli	Sorghum bicolor

Protein Variants

Protein Variants	Comment	Organism
E410A	glucosylation of mandelonitrile is 225fold reduced compared to wild-type enzyme	Sorghum bicolor
R201A	glucosylation of mandelonitrile is 20fold reduced compared to wild-type enzyme	Sorghum bicolor
S391A	glucosylation of mandelonitrile is 185fold reduced compared to wild-type enzyme	Sorghum bicolor

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.8	-	UDP-glucose	pH 7.5, 30°C, cosubstrate: mandelonitrile	Sorghum bicolor

Organism

Organism	UniProt	Comment	Textmining
Sorghum bicolor	Q9SBL1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + mandelonitrile	specific for UDP-glucose	Sorghum bicolor	UDP + mandelonitrile beta-D-glucoside	-	?

Synonyms

Synonyms	Comment	Organism
cyanohydrin glycosyltransferase	-	Sorghum bicolor
UGT85B1	-	Sorghum bicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.9	-	UDP-glucose	pH 7.5, 30°C, cosubstrate: mandelonitrile	Sorghum bicolor

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Release 2023.1 (January 2023)