Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | requirement, 5 mM | Sorghum bicolor |
General Stability | Organism |
---|---|
stable to freezing, DEAE-chromatography decreases stability to freezing | Sorghum bicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
UDPglucose | - |
Sorghum bicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by Ca2+ or Mg2+ | Sorghum bicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + (S)-4-hydroxymandelonitrile | Sorghum bicolor | involved in dhurrin biosynthesis | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | dhurrin is the cyanogenic glucoside characteristic of Sorghum species | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
Sudan gras hybrid, Sordan-70, cv. Moench | - |
Purification (Comment) | Organism |
---|---|
77fold, partial | Sorghum bicolor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | etiolated | Sorghum bicolor | - |
seedling | shoots | Sorghum bicolor | - |
shoot | shoots of etiolated seedlings | Sorghum bicolor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.76 | - |
- |
Sorghum bicolor |
Storage Stability | Organism |
---|---|
0°C, unstable at | Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | reaction is specific for an aromatic group, but not for a p-hydroxy substituent on the aromatic group, no substrates: catechol, resorcinol, phloroglucinol, fructose, glucose, dhurrin, acetone cyanohydrin, acetaldehyde cyanohydrin, GDPglucose, TDPglucose, ADPglucose | Sorghum bicolor | additional information | no product: taxiphyllin | ? | |
UDP-alpha-D-glucose + phenol | very poor substrate | Sorghum bicolor | UDP + phenyl beta-D-glucopyranoside | - |
? | |
UDP-glucose + 4-hydroxybenzaldehyde | poor substrate, mixture of 4-hydroxybenzaldehyde with NaCN: good substrate | Sorghum bicolor | UDP + 4-formylphenyl beta-D-glucopyranoside | - |
? | |
UDPglucose + (S)-4-hydroxymandelonitrile | stereospecific for the S-enantiomer | Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | identical with dhurrin | ? | |
UDPglucose + (S)-4-hydroxymandelonitrile | involved in dhurrin biosynthesis | Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | dhurrin is the cyanogenic glucoside characteristic of Sorghum species | ? | |
UDPglucose + (S)-mandelonitrile | as good as 4-hydroxymandelonitrile | Sorghum bicolor | UDP + (S)-mandelonitrile beta-D-glucoside | - |
? | |
UDPglucose + 4-hydroxybenzoic acid | poor substrate | Sorghum bicolor | UDP + glucosyl-4-hydroxybenzoate | - |
? | |
UDPglucose + 4-hydroxybenzyl alcohol | at 36% the rate of 4-hydroxymandelonitrile | Sorghum bicolor | UDP + 4-hydroxybenzyl glucoside | - |
? | |
UDPglucose + hydroquinone | at 41% the rate of 4-hydroxymandelonitrile | Sorghum bicolor | UDP + hydroquinone glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sorghum bicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.5 | - |
Sorghum bicolor |