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Literature summary for 2.4.1.85 extracted from

  • Reay, P.F.; Conn, E.E.
    The purification and properties of a uridine diphosphate glucose: aldehyde cyanohydrin beta-glucosyltransferase from sorghum seedlings (1974), J. Biol. Chem., 249, 5826-5830.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol requirement, 5 mM Sorghum bicolor

General Stability

General Stability Organism
stable to freezing, DEAE-chromatography decreases stability to freezing Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
UDPglucose
-
Sorghum bicolor

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activated by Ca2+ or Mg2+ Sorghum bicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + (S)-4-hydroxymandelonitrile Sorghum bicolor involved in dhurrin biosynthesis UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside dhurrin is the cyanogenic glucoside characteristic of Sorghum species ?

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
Sudan gras hybrid, Sordan-70, cv. Moench
-

Purification (Commentary)

Purification (Comment) Organism
77fold, partial Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
seedling etiolated Sorghum bicolor
-
seedling shoots Sorghum bicolor
-
shoot shoots of etiolated seedlings Sorghum bicolor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.76
-
-
Sorghum bicolor

Storage Stability

Storage Stability Organism
0°C, unstable at Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information reaction is specific for an aromatic group, but not for a p-hydroxy substituent on the aromatic group, no substrates: catechol, resorcinol, phloroglucinol, fructose, glucose, dhurrin, acetone cyanohydrin, acetaldehyde cyanohydrin, GDPglucose, TDPglucose, ADPglucose Sorghum bicolor additional information no product: taxiphyllin ?
UDP-alpha-D-glucose + phenol very poor substrate Sorghum bicolor UDP + phenyl beta-D-glucopyranoside
-
?
UDP-glucose + 4-hydroxybenzaldehyde poor substrate, mixture of 4-hydroxybenzaldehyde with NaCN: good substrate Sorghum bicolor UDP + 4-formylphenyl beta-D-glucopyranoside
-
?
UDPglucose + (S)-4-hydroxymandelonitrile stereospecific for the S-enantiomer Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside identical with dhurrin ?
UDPglucose + (S)-4-hydroxymandelonitrile involved in dhurrin biosynthesis Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside dhurrin is the cyanogenic glucoside characteristic of Sorghum species ?
UDPglucose + (S)-mandelonitrile as good as 4-hydroxymandelonitrile Sorghum bicolor UDP + (S)-mandelonitrile beta-D-glucoside
-
?
UDPglucose + 4-hydroxybenzoic acid poor substrate Sorghum bicolor UDP + glucosyl-4-hydroxybenzoate
-
?
UDPglucose + 4-hydroxybenzyl alcohol at 36% the rate of 4-hydroxymandelonitrile Sorghum bicolor UDP + 4-hydroxybenzyl glucoside
-
?
UDPglucose + hydroquinone at 41% the rate of 4-hydroxymandelonitrile Sorghum bicolor UDP + hydroquinone glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.5
-
Sorghum bicolor