Cloned (Comment) | Organism |
---|---|
DPMS (KB-3) gene cloned into the pcDNA3.1/His A vector at the KpnI and XhoI site and transformed into DH5alpha cells. Cloned into Xho I and BamH I sites of pEGFP-N1 to obtain pEGFP-N1-DPMS overexpression plasmid. Transfection of capillary endothelial cells with pEGFP-N1-DPMS overexpression plasmid and pEGFP-N1 vector | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | DPMS has one membrane spanning region | Bos taurus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, recombinant His-tag DPMS, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tag fusion protein purified using ProBond purification system, more than 97% pure | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal medulla | capillary endothelial cell | Bos taurus | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, recombinant His-tag DPMS, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
DPMS | - |
Bos taurus |
mannosylphospho dolichol synthase | - |
Bos taurus |
Organism | Comment | Expression |
---|---|---|
Bos taurus | capillary endothelial cells harboring DPMS overexpressing plasmid express DPMS higher than pEGFP-N1 vector and the parental cells | up |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpression of DPMS accelerates angiogenesis, a higher rate of cellular proliferation in DPMS overexpressing cells compared to the pEGFP-N1 vector and the parental cells | Bos taurus |