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Literature summary for 2.4.1.83 extracted from
- Solubilization and properties of polyprenyl phosphate: GDP-D-mannose mannosyl transferase (1979), Arch. Biochem. Biophys., 198, 117-123.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | - |
Acanthamoeba castellanii |  |
| GDP | 0.01 mM, 81% inhibition | Acanthamoeba castellanii | |
| GDP-D-glucose | 0.01 mM, 50% inhibition | Acanthamoeba castellanii | |
| GMP | 1 mM, 84% inhibition; competitive | Acanthamoeba castellanii | |
| HgCl2 | - |
Acanthamoeba castellanii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
GDPmannose | - |
Acanthamoeba castellanii | |
| 0.0033 | - |
dolichyl phosphate | - |
Acanthamoeba castellanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | less efficient in activation than Mn2+ | Acanthamoeba castellanii | |
| Mg2+ | less efficient than Mn2+ in activation | Acanthamoeba castellanii | |
| Mn2+ | divalent cation required, maximal activity with Mn2+ | Acanthamoeba castellanii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba castellanii | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Acanthamoeba castellanii | - |
| cell culture | encysting | Acanthamoeba castellanii | - |
| cyst | - |
Acanthamoeba castellanii | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, loss of a third of the activity after 2 weeks | Acanthamoeba castellanii |
| -70°C, 2 weeks, no loss of activity | Acanthamoeba castellanii |
| 0°C, 3 days, 50% loss of activity | Acanthamoeba castellanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDPmannose + dolichyl phosphate | - |
Acanthamoeba castellanii | GDP + dolichyl D-mannosyl phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
- |
Acanthamoeba castellanii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 35 | 15°C: about 60% of maximal activity, 35°C: about 30% of maximal activity | Acanthamoeba castellanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Acanthamoeba castellanii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8 | pH 5.8: about 35% of maximal activity, pH 8.0: about 60% of maximal activity | Acanthamoeba castellanii |
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