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Literature summary for 2.4.1.83 extracted from

  • Tomita, Y.; Motokawa, Y.
    GDPmannose dolicholphosphate mannosyltransferase of chicken liver mitochondria (1985), Biochim. Biophys. Acta, 842, 176-183.
    View publication on PubMed

General Stability

General Stability Organism
in combination dolichyl phosphate and phospholipids are effective in stabilization Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00043
-
GDPmannose
-
Gallus gallus
0.0143
-
dolichyl phosphate
-
Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal stimulation at 3 mM Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Reaction

Reaction Comment Organism Reaction ID
GDP-alpha-D-mannose + dolichyl phosphate = GDP + dolichyl beta-D-mannosyl phosphate sequential mechanism Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Storage Stability

Storage Stability Organism
-40°C, stable for about a month with 20% loss of activity Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDPmannose + dolichyl phosphate
-
Gallus gallus GDP + dolichyl D-mannosyl phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
20 min, complete inactivation, in presence of mitochondrial extract 40% loss of activity, stable for 30 min in presence of sphingomyelin and dolichyl phosphate Gallus gallus
40
-
20 min, 50% loss of activity in presence of sphingomyelin and dolichyl phosphate Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Gallus gallus