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Literature summary for 2.4.1.81 extracted from

  • Kim, J.H.; Kim, B.G.; Park, Y.; Ko, J.H.; Lim, C.E.; Lim, J.; Lim, Y.; Ahn, J.
    Characterization of flavonoid 7-O-glucosyltransferase from Arabidopsis thaliana (2006), Biosci. Biotechnol. Biochem., 70, 1471-1477.
    View publication on PubMed

Application

Application Comment Organism
synthesis biotransformation of eriodictyol using Escherichi coli expressing AtGT-2 Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a glutathione S-transferase fusion protein Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
eriodictyol pH 7.0 Arabidopsis thaliana
0.0263
-
kaempferol pH 7.0 Arabidopsis thaliana
0.0353
-
quercetin pH 7.0 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54800
-
x * 54800, calculated from sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8VZE9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower high expression Arabidopsis thaliana
-
leaf high expression Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + apigenin 29.6% of the activity with eriodictyol Arabidopsis thaliana UDP + apigenin 7-O-beta-D-glucoside
-
?
UDP-alpha-D-glucose + kaempferol 78.9% of the activity with eriodictyol Arabidopsis thaliana UDP + kaempferol 7-O-beta-D-glucoside
-
?
UDP-alpha-D-glucose + quercetin 64.1% of the activity with eriodictyol Arabidopsis thaliana UDP + quercetin 7-O-beta-D-glucoside
-
?
UDP-glucose + eriodictyol
-
Arabidopsis thaliana UDP + 7-O-beta-D-glucosyl-eriodictyol
-
?
UDP-glucose + luteolin 92.1% of the activity with eriodictyol Arabidopsis thaliana UDP + 7-O-beta-D-glucosyl-luteolin
-
?
UDP-glucose + naringenin 71.6% of the activity with eriodictyol Arabidopsis thaliana UDP + 7-O-beta-D-glucosyl-naringenin
-
?

Subunits

Subunits Comment Organism
? x * 54800, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtGT-2
-
Arabidopsis thaliana