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Literature summary for 2.4.1.80 extracted from

  • Gouaze, V.; Yu, J.Y.; Bleicher, R.J.; Han, T.Y.; Liu, Y.Y.; Wang, H.; Gottesman, M.M.; Bitterman, A.; Giuliano, A.E.; Cabot, M.C.
    Overexpression of glucosylceramide synthase and P-glycoprotein in cancer cells selected for resistance to natural product chemotherapy (2004), Mol. Cancer Ther., 3, 633-639.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in cancer cell lines selected for resistance to natural product chemotherapy, i.e. cell lines KB-3-1, KB-V.01, KB-V.1, KB-V1, KB-A.05, MCF-7, and MCF-7-AdrR, cell lines show different recombinant enzyme activity, which is higherin cells resistant to natural product anticancer agents, e.g. vinblastine and adriamycin, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00017
-
recombinant enzyme in cell extract of cell line KB-3-1 Homo sapiens
0.00021
-
recombinant enzyme in cell extract of cell line KB-A1 Homo sapiens
0.00024
-
recombinant enzyme in cell extract of cell line KB-V.1 Homo sapiens
0.00025
-
recombinant enzyme in cell extract of cell line KB-A.05 Homo sapiens
0.00036
-
recombinant enzyme in cell extract of cell line KB-V1 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + C6-ceramide
-
Homo sapiens UDP + glucosyl-C6-ceramide
-
?

Synonyms

Synonyms Comment Organism
glucosylceramide synthase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Homo sapiens