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Literature summary for 2.4.1.79 extracted from

  • Lockney, M.W.; Sweely, C.S.
    Characterization of a glycosphingolipid beta-N-acetylgalactosaminyl-transferase activity in cultured hamster (nil) cells (1982), Biochim. Biophys. Acta, 712, 234-241.
    View publication on PubMed
Search for more literature for 2.4.1.79

Activating Compound

Activating Compound Comment Organism Structure
Detergents
-
 Mesocricetus auratus
sodium taurodeoxycholate greatest activation, 1 mg/ml Mesocricetus auratus

Inhibitors

Inhibitors Comment Organism Structure
Globotetraosylceramide
-
 Mesocricetus auratus
II3-alpha-N-Acetylneuraminyl-lactosylceramide
-
 Mesocricetus auratus
additional information no effect: lactosylceramide Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 UDP-N-acetyl-D-galactosamine
-
 Mesocricetus auratus
0.42
-
 globotriosylceramide
-
 Mesocricetus auratus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required for maximal activity, 4 mM Mesocricetus auratus
additional information Mg2+ cannot replace Mn2+ Mesocricetus auratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + globotriosylceramide Mesocricetus auratus glycosphingolipid metabolism UDP + globotetraosylceramide
-
 ?

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast cultured hamster cells, NIL-8 Mesocricetus auratus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + globotriosylceramide highly acceptor specific, does not act on globotetraosylceramide or lactosylceramide Mesocricetus auratus UDP + globotetraosylceramide the product has a terminal beta-N-acetylgalactosamine residue ?
UDP-N-acetyl-D-galactosamine + globotriosylceramide glycosphingolipid metabolism Mesocricetus auratus UDP + globotetraosylceramide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mesocricetus auratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 8 maximal activity in 2-(N-morpholino)ethanesulfonic acid Mesocricetus auratus