Cloned (Comment) | Organism |
---|---|
unspase, genetic library construction, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged unspase in Escherichia coli strain XL-1 Blue | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.12 | - |
sucrose | pH 6.5, 37°C, recombinant enzyme | uncultured bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | uncultured bacterium | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | B8Y3Y0 | isolated from soil collected from a sugar refinery in Wuming, Guangxi Province, China | - |
Purification (Comment) | Organism |
---|---|
recombinant unspase from Escherichia coli strain XL-1 Blue by affinity chromatography | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the unspase is a sucrose phosphorylase able to catalyze the transglycosylation of different monomeric sugars, L-arabinose, D-fructose and L-sorbose, resulting in a 38% conversion rate | uncultured bacterium | ? | - |
? | |
sucrose + L-arabinose | - |
uncultured bacterium | D-fructose + ? | the enzyme transglucosylated L-arabinose even in phosphate buffer | ? | |
sucrose + L-sorbose | - |
uncultured bacterium | D-fructose + ? | - |
? | |
sucrose + phosphate | - |
uncultured bacterium | D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
unspase | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
purified enzyme, 10 min, inactivation | uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.19 | - |
sucrose | pH 6.5, 37°C, recombinant enzyme | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | activity drops sharply below pH 5.5 | uncultured bacterium |
General Information | Comment | Organism |
---|---|---|
additional information | compared with Bisp, the sucrose phosphorylase from Bifidobacterium adolescentis, unspase has two deleted regions in its C-terminal. These deleted regions are probably equivalent to the important five-stranded anti-parallel beta-sheet domain in sucrose phosphorylase | uncultured bacterium |
physiological function | sucrose phosphorylase is an important enzyme mainly involved in the generic starch and sucrose pathways | uncultured bacterium |