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Literature summary for 2.4.1.7 extracted from

  • Renirie, R.; Pukin, A.; Van Lagen, B.; Franssen, M.
    Regio- and stereoselective glucosylation of diols by sucrose phosphorylase using sucrose or glucose 1-phosphate as glucosyl donor (2010), J. Mol. Catal. B, 67, 219-224.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
D-fructose
-
Leuconostoc mesenteroides
phosphate
-
Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + phosphate Leuconostoc mesenteroides
-
D-fructose + alpha-D-glucose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + (R,S)-1,2-butandiol regioselective glucosylation Leuconostoc mesenteroides phosphate + 2-O-(alpha-D-glucopyranosyl)-1,2-butandiol
-
?
alpha-D-glucose 1-phosphate + glycerol The glucoside yield is higher when sucrose is used as a donor rather than alpha-D-glucose 1-phosphate, due to the fact that the released phosphate is a stronger inhibitor of the enzyme in case of alpha-D-glucose 1-phosphate than the released fructose in case of sucrose Leuconostoc mesenteroides phosphate + 2-O-alpha-D-glucopyranosyl-sn-glycerol
-
?
additional information regio- and stereoselective glucosylation of diols by sucrose phosphorylase using sucrose or glucose 1-phosphate as glucosyl donor, stereochemistry of products from glucosyl transfer and phosphorolysis an hydrolysis reactions, NMR analysis, overview. Mono-alcohols are not accepted as substrates but several 1,2-diols are readily glucosylated, proving that the vicinal diol unit is crucial for activity. The smallest substrate that is accepted for glucosylation appears to be ethylene glycol, it is converted to the monoglucoside by 69%. No activity with (R,S)-3-amino-1,2-propanediol (R,S)-3-chloro-1,2-propanediol, (R,S)-1-thioglycerol, and (R,S)-glyceraldehyde Leuconostoc mesenteroides ?
-
?
sucrose + (R)-1,2-propanediol regioselective glucosylation Leuconostoc mesenteroides D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-propanediol
-
?
sucrose + (R,S)-1,2-butandiol regioselective glucosylation, sucrose is the preferred glucosyl donor with 1,2-butandiol compared to alpha-D-glucose 1-phosphate Leuconostoc mesenteroides D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-butandiol
-
?
sucrose + (R,S)-1,2-propanediol regioselective glucosylation Leuconostoc mesenteroides D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-propanediol
-
?
sucrose + (R,S)-3-methoxy-1,2-propanediol regioselective glucosylation Leuconostoc mesenteroides D-fructose + 3-methoxy-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol
-
?
sucrose + (S)-1,2-propanediol regioselective glucosylation Leuconostoc mesenteroides D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-propanediol
-
?
sucrose + 3-tert-butoxy-1,2-propanediol regioselective glucosylation Leuconostoc mesenteroides D-fructose + 3-tert-butoxy-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol
-
?
sucrose + ethylene glycol
-
Leuconostoc mesenteroides D-fructose + 2-O-alpha-D-glucopyranosyl-ethylene glycol
-
?
sucrose + glycerol regio- and stereoselective glucosylation. The glucoside yield is higher when sucrose is used as a donor rather than alpha-D-glucose 1-phosphate, due to the fact that the released phosphate is a stronger inhibitor of the enzyme in case of alpha-D-glucose 1-phosphate than the released fructose in case of sucrose Leuconostoc mesenteroides D-fructose + 2-O-alpha-D-glucopyranosyl-sn-glycerol
-
?
sucrose + phosphate
-
Leuconostoc mesenteroides D-fructose + alpha-D-glucose 1-phosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
assay at Leuconostoc mesenteroides