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Literature summary for 2.4.1.7 extracted from

  • Kasperowicz, A.; Stan-Glasek, K.; Guczynska, W.; Piknova, M.; Pristas, P.; Nigutova, K.; Javorsky, P.; Michalowski, T.
    Sucrose phosphorylase of the rumen bacterium Pseudobutyrivibrio ruminis strain A (2009), J. Appl. Microbiol., 107, 812-820.
    View publication on PubMed
Search for more literature for 2.4.1.7

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree Pseudobutyrivibrio ruminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, calculated Km of D-glucose 1-phosphate formation and release of D-fructose are 3.88 mM and 5.56 mM Pseudobutyrivibrio ruminis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 gel filtration Pseudobutyrivibrio ruminis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + phosphate Pseudobutyrivibrio ruminis
-
 D-fructose + D-glucose 1-phosphate
-
 ?
sucrose + phosphate Pseudobutyrivibrio ruminis A
-
 D-fructose + D-glucose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudobutyrivibrio ruminis
-
 isolated from the rumen of sheep in Poland
-
Pseudobutyrivibrio ruminis A
-
 isolated from the rumen of sheep in Poland
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 10fold, partially by gel filtration Pseudobutyrivibrio ruminis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + phosphate
-
 Pseudobutyrivibrio ruminis D-fructose + D-glucose 1-phosphate
-
 ?
sucrose + phosphate
-
 Pseudobutyrivibrio ruminis A D-fructose + D-glucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Pseudobutyrivibrio ruminis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudobutyrivibrio ruminis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 activity range, inactive above 55°C, profile, overview Pseudobutyrivibrio ruminis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pseudobutyrivibrio ruminis

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 activity range, no activity below pH 5.0, profile, overview Pseudobutyrivibrio ruminis