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Literature summary for 2.4.1.7 extracted from

  • Abada, E.; Osman, M.; Lee, J.; Kim, D.
    Molecular cloning of the gene 1355SPase encoding a sucrose phosphorylase from the bacterium Leuconostoc mesenteroides B-1355 (2008), Biotechnology, 7, 463-468.
No PubMed abstract available
Search for more literature for 2.4.1.7

Cloned(Commentary)

Cloned (Comment) Organism
gene 1355SPase, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain DH5alpha Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
D295E site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme Leuconostoc mesenteroides
D295N site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56600
-
 x * 56600, about, sequence calculation, x * 60600, recombinant His-tagged enzyme, SDS-PAGE Leuconostoc mesenteroides
60600
-
 x * 56600, about, sequence calculation, x * 60600, recombinant His-tagged enzyme, SDS-PAGE Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + phosphate Leuconostoc mesenteroides
-
 D-fructose + D-glucose 1-phosphate
-
 ?
sucrose + phosphate Leuconostoc mesenteroides NRRL B-1355
-
 D-fructose + D-glucose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides B0F411 gene 1355SPase
-
Leuconostoc mesenteroides NRRL B-1355 B0F411 gene 1355SPase
-

Purification (Commentary)

Purification (Comment) Organism
reconbinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography Leuconostoc mesenteroides

Source Tissue

Source Tissue Comment Organism Textmining
additional information constitutive enzyme Leuconostoc mesenteroides
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
 purified enzyme, substrate sucrose Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows high substrate specificity towards glucosyl donors accepting only sucrose, glucose 1-phosphate, and glucose 1-fluoride, but a broad substrate specificity towards glycosyl acceptors, overview. No activity with melibiose, melezitose, and raffinose Leuconostoc mesenteroides ?
-
 ?
additional information the enzyme shows high substrate specificity towards glucosyl donors accepting only sucrose, glucose 1-phosphate, and glucose 1-fluoride, but a broad substrate specificity towards glycosyl acceptors, overview. No activity with melibiose, melezitose, and raffinose Leuconostoc mesenteroides NRRL B-1355 ?
-
 ?
sucrose + phosphate
-
 Leuconostoc mesenteroides D-fructose + D-glucose 1-phosphate
-
 ?
sucrose + phosphate
-
 Leuconostoc mesenteroides NRRL B-1355 D-fructose + D-glucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 56600, about, sequence calculation, x * 60600, recombinant His-tagged enzyme, SDS-PAGE Leuconostoc mesenteroides

Synonyms

Synonyms Comment Organism
1355SPase
-
 Leuconostoc mesenteroides
SPase
-
 Leuconostoc mesenteroides
sucrose: orthophosphate, alpha-D-glucosyltransferase
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Leuconostoc mesenteroides